Table 1.
Primer sequences used for amplification of POMC gene.
| Primers | Sequences (5′-3′) | Tm(°C) | Product size (bp) |
|---|---|---|---|
| POMC1-F | TCTCCAAGGGCCATCCAGAG | 59.5 | 1,748 |
| POMC1-R | TCCCTGATTTCCTGGTTTGTC | 55.6 | |
| POMC2-F | AGGCGGAGTGATACCTTGAGC | 59.5 | 984 |
| POMC2-R | CTTCCCTTCCTCTCGTTCCA | 57.4 | |
| POMC3-F | GGAGAGCATCCGCAAGTACG | 59.5 | |
| POMC3-R | TTGGGAGTAACCTATGCTGAAGT | 56.0 | 1,275 |
| POMC4-F | GCTGCCAACCTCCATACCTAATAC | 59.6 | |
| POMC4-R | TGTCATGTAATCCGGGTTTAGG | 55.8 | 1,472 |
Owing to the complexity of POMC gene, fragments were divided into 4 segments in the sequencing process and primers were designed respectively.