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. Author manuscript; available in PMC: 2020 Oct 30.
Published in final edited form as: Methods Mol Biol. 2020;2143:83–96. doi: 10.1007/978-1-0716-0585-1_7

Figure 3: Fabrication of microfluidics.

Figure 3:

a. Design of two chamber microfluidics. The microgoove is 10 μm wide, 350 μm long. The size of microfluidic device is 22×22 mm, which fits the square glass coverslips.

b. Picture of foil cup.

c. Picture of the master mold.

d. Picture of PDMS mold after debubbling.

e. Picture of PDMS mold after cutting out the square block.

f. Picture of punching holes over the circle reserviors.

g. Picture of individual microfluidic device.

h. Picture of individual microfluidic device after cutting corners.

i. Picture of PDMS microfluidics.