Table 9.
Concentration of phytate (InsP6, InsP isomers, and myo-inositol [μmol/g DM]) in the digesta of the small intestine of broiler chickens fed the experimental diets (least square means, pooled SEM; n = 5 pens per diet).
| Meal | Level | Phy | Myo-inositol | InsP31 | Ins(…)P4 |
Ins(…)P5 |
InsP6 | |||
|---|---|---|---|---|---|---|---|---|---|---|
| (1,2,3,4) | (1,2,5,6) | (1,2,3,4,5) | (1,2,4,5,6) | (1,2,3,4,6) | ||||||
| RSM | 1 | − | 3.3 | 0.3 | 0.4 | 0.6 | 2.5 | 2.6 | 1.0 | 46.9 |
| 2 | − | 2.9 | <LOQ | 0.3 | 0.7 | 2.8 | 3.0 | 1.1 | 50.6 | |
| 1 | + | 10.6 | 10.0 | 7.6 | 4.7 | 4.4 | 1.5 | <LOQ | 11.7 | |
| 2 | + | 10.6 | 7.2 | 8.7 | 5.2 | 6.5 | 2.1 | 0.2 | 15.8 | |
| SBM | 1 | − | 4.0 | <LOQ | 0.3 | 0.6 | 2.3 | 2.5 | 0.8 | 46.2 |
| 2 | − | 6.2 | <LOQ | 0.4 | 0.6 | 2.3 | 2.3 | 0.8 | 47.4 | |
| 1 | + | 17.0 | 9.6 | 6.6 | 4.0 | 3.1 | 1.0 | nd | 8.1 | |
| 2 | + | 19.4 | 5.3 | 6.3 | 3.4 | 3.8 | 1.2 | nd | 11.3 | |
| SFM | 1 | − | 3.2 | 0.3 | 0.7 | 0.9 | 4.1 | 3.2 | 1.0 | 53.8 |
| 2 | − | 4.4 | 0.3 | 0.8 | 1.0 | 4.6 | 3.3 | 1.1 | 53.5 | |
| 1 | + | 12.8 | 10.3 | 9.5 | 5.9 | 6.8 | 2.1 | 0.2 | 16.2 | |
| 2 | + | 11.7 | 6.1 | 8.9 | 5.0 | 8.6 | 2.6 | 0.3 | 22.9 | |
| SEM | 1.34 | 0.70 | 0.71 | 0.42 | 0.78 | 0.26 | 0.05 | 2.40 | ||
| 2-way interactions | ||||||||||
| Meal × Phy | ||||||||||
| RSM | − | 3.1c | - | 0.4c | 0.6c | 2.6d | 2.8 | 1.0 | 48.7 | |
| + | 10.6b | 8.6 | 8.2a | 5.0a | 5.5b | 1.8 | - | 13.8 | ||
| SBM | − | 5.1c | - | 0.4c | 0.6c | 2.3d | 2.4 | 0.8 | 46.8 | |
| + | 18.2a | 7.4 | 6.4b | 3.7b | 3.5c,d | 1.1 | - | 9.7 | ||
| SFM | − | 3.8c | 0.3 | 0.8c | 0.9c | 4.4b,c | 3.2 | 1.0 | 53.7 | |
| + | 12.2b | 8.2 | 9.2a | 5.4a | 7.7a | 2.3 | 0.2 | 19.5 | ||
| SEM | 1.07 | 0.49 | 0.56 | 0.32 | 0.62 | 0.23 | 0.04 | 2.02 | ||
| Main effects | ||||||||||
| RSM | 6.8 | - | 4.3 | 2.8 | 4.0 | 2.3b | - | 31.2b | ||
| SBM | 11.7 | - | 3.4 | 2.2 | 2.9 | 1.8c | - | 28.3c | ||
| SFM | 8.0 | 4.2 | 5.0 | 3.2 | 6.0 | 2.8a | 0.6 | 36.6a | ||
| SEM | 0.90 | 0.35 | 0.47 | 0.26 | 0.53 | 0.21 | 0.04 | 2.02 | ||
| 1 | 8.5 | - | 4.2 | 2.8 | 3.9b | 2.2b | - | 30.5b | ||
| 2 | 9.2 | - | 4.2 | 2.6 | 4.8a | 2.4a | - | 33.6c | ||
| SEM | 0.84 | - | 0.43 | 0.24 | 0.50 | 0.21 | - | 1.97 | ||
| − | 4.0 | - | 0.5 | 0.7 | 3.1 | 2.8a | 1.0 | 49.8a | ||
| + | 13.7 | 8.1 | 7.9 | 4.7 | 5.5 | 1.8b | - | 14.3b | ||
| SEM | 0.84 | 0.35 | 0.43 | 0.24 | 0.62 | 0.21 | 0.04 | 1.97 | ||
| P value | ||||||||||
| Meal | <0.001 | 0.235 | 0.003 | 0.001 | <0.001 | <0.001 | <0.001 | <0.001 | ||
| Phy | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | <0.001 | ||
| Level | 0.283 | <0.001 | 0.874 | 0.518 | 0.018 | 0.009 | 0.002 | <0.001 | ||
| Meal × level | 0.237 | 0.528 | 0.656 | 0.354 | 0.580 | 0.097 | 0.378 | 0.716 | ||
| Phy × level | 0.676 | 0.006 | 0.978 | 0.390 | 0.100 | 0.078 | 0.428 | 0.075 | ||
| Meal × Phy | 0.002 | 0.892 | 0.025 | 0.023 | 0.049 | 0.288 | 0.365 | 0.365 | ||
| Meal × Phy × level | 0.641 | -2 | 0.491 | 0.416 | 0.818 | 0.884 | -2 | 0.283 | ||
Level 1 = 150 g/kg SBM, 100 g/kg RSM, 150 g/kg SFM; level 2 = 300 g/kg SBM, 200 g/kg RSM, 300 g/kg SFM. − = without added phytase; + = added phytase; values in the same column and within the same subheading not sharing the same superscript letter are significantly different (P ≤ 0.05).
Abbreviations: <LOQ, not quantifiable in most samples; nd, not detectable in most samples; Phy, phytase; RSM, rapeseed meal; SBM, soybean meal; SFM, sunflower meal.
At least one of the following isomers: Ins(1,2,6)P3, Ins(1,4,5)P3, Ins(2,4,5)P3.
No P values given due to values under the limit of quantification or detection.