Table 2.
Primer used for quantitative real-time PCR.
| Target gene | Primer | Primer sequence (5′-3′) | Accession No. |
|---|---|---|---|
| β-Actin | Forward | TCCCTGGAGAAGAGCTATGAA | NM_205518.1 |
| Reverse | CAGGACTCCATACCCAAGAAAG | ||
| IL-10 | Forward | CCAGGGACGATGAACTTAACA | NM_001004414.2 |
| Reverse | GATGGCTTTGCTCCTCTTCT | ||
| IL-1β | Forward | CTCACAGTCCTTCGACATCTTC | XM_015297469.1 |
| Reverse | CGGTACATACGAGATGGAAACC | ||
| IL-6 | Forward | GATCCGGCAGATGGTGATAAA | NM_204628.1 |
| Reverse | GAGGATTGTGCCCGAACTAAA | ||
| TNF-α | Forward | GACAGCCTATGCCAACAAGTA | AY765397.1 |
| Reverse | TCCACATCTTTCAGAGCATCAA | ||
| Cyt C | Forward | GGAGATATTGAGAAGGGCAAGAA | XM_015281453.2 |
| Reverse | ATCATCTTTGTTCCTGGGATGT | ||
| Chop | Forward | GCACAGCCCATTTCTGTTTC | XM_015273173.2 |
| Reverse | TGCCATCCCATTCTGCTAAG | ||
| Grp78 | Forward | GTTACTGTGCCAGCCTACTT | NM_205491.1 |
| Reverse | CCGCTTCGCTTTCTCTACTT | ||
| Caspase 9 | Forward | GACCTGCTAACCATGCTACTT | XM_424580.6 |
| Reverse | TTCCACTGAATCCTCCAATCC | ||
| Caspase 3 | Forward | TCCCTGGTTCCAAAGGAATG | XM_015276122.2 |
| Reverse | AGTAGCCTGGAGCAGTAGAA | ||
| Caspase 7 | Forward | TGCAAAGCCAGACAGAAGTAG | XM_025151846.1 |
| Reverse | GGTCCATCGGTGCCATAAAT | ||
| ACOX1 | Forward | ACTGAGCTGTGTCTCTTGTATG | XM_015295164.2 |
| Reverse | GCTTCAGGTGTTTGTGGAAAG | ||
| CPT1 | Forward | GAGAAGAGTGCAGTGAGAAGAG | XM_015286798.2 |
| Reverse | CCAGCCACAGAAGTAGAGTAAG | ||
| FASN | Forward | CCTGGAGATGTGGAGTATGTTG | NM_205155.3 |
| Reverse | TCAAGGAGCCATCGTGTAAAG | ||
| PPARα | Forward | GATGCTGCGTGAAGTGAAATG | XM_025150258.1 |
| Reverse | CTGGTGAAAGGGTGTCTGTTAT | ||
| ApoB | Forward | ATTCCTGACTTGAAGATACCAGAG | NM_001044633.1 |
| Reverse | GTTCGCAGATGCTGTAGTATTATG | ||
| MTP | Forward | AGGAGAGGGAAAGCAGAAATG | NM_001109784.2 |
| Reverse | ATTTCCCATTACCCGCAGTAG | ||
| VTG II | Forward | TCATCTGCCTCCTCTCCTAATC | NM_001031276.1 |
| Reverse | ACTGCTCCTACTACTGCTACTT | ||
| ApoVLDL II | Forward | CTCACTAAACTGGCGGAACA | M25774.1 |
| Reverse | GCCAAGTCATTCAGGAGGAA |