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Screening of reaction temperature and reaction time for NDV detection by RF-RT-RAA. Reaction time was set to 26 min, (A–E) 37°C to 41°C, respectively. As per the peak time and fluorescence value of the amplification curve, 38°C was determined as the optimum reaction temperature and 26 min was determined as the optimum reaction time. Abbreviations: NDV, Newcastle disease virus; RF-RT-RAA, real-time fluorescence-based reverse transcription recombinase–aided amplification.
