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Specificity of RF-RT-RAA in NDV detection. 1–4: Negative control, ILTV, AIV and IBV, respectively; 5: NDV. Bands appeared in the NDV test area; no fluorescence curve for other viral nucleic acids and negative control except for NDV. Abbreviations: AIV, avian influenza virus; IBV, infectious bronchitis virus; ILTV, infectious laryngotracheitis virus; NDV, Newcastle disease virus; RF-RT-RAA, real-time fluorescence-based reverse transcription recombinase–aided amplification.
