Table 1.
Primers | Sequence (5′-3′) | Gene localization |
---|---|---|
NDV-1-F | TGATTCGTGGACAGACAGTAAGGAAGACTCGG | 3431-3466 |
NDV-1-R | TGAAAGTAACTCGTGCTTGGGATTATCGCTG | 3542-3573 |
NDV-2-F | TGGACGTGGTCCCGAAGAGCCCGTTAGTCA | 3930-3960 |
NDV-2-R | CGAGCACATCACTGAGCCCGACAGATAAAT | 4090-4120 |
NDV-3-F | CAGCAAGCCATCTTATCTATCAAAGTGTCAACATC | 1108-1143 |
NDV-3-R | TGTCCCTACTGTGAGAACTCTGCCTTCGGC | 1227-1257 |
NDV-4-F | ACGTCTTTATTACTCCTGAGCTTGTCATTGTGA | 9008-9041 |
NDV-4-R | TGATTACCTAAGTCCTTTGCCAGAGCATCTACT | 9227-9260 |
Abbreviations: NDV, Newcastle disease virus; RT-RAA, reverse transcription recombinase–aided amplification.