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Primer sequence of gene
| Gene | Forward primer sequence (5’–3’) | Reverse primer sequence (5’–3’) |
|---|---|---|
| GAPDH | ACCCAGAAGACTGTGGATGG | CACATTGGGGGTAGGAACAC |
| ALP | CCAGCAGGTTTCTCTCTTGG | GGGATGGAGGAGAGAAGGTC |
| COL-I | GAGCGGAGAGTACTGGATCG | GTTCGGGCTGATGTACCAGT |
| OPN | TCTGATGAGACCGTCACTGC | AGGTCCTCATCTGTGGCATC |
| OPG | TCCTGGCACCTACCTAAA | CACCTGAGAAGAACCCATC |
| OC | GGACCATCTTTCTGCTCACTCTG | TTCACTACCTTATTGCCCTCCTG |
| OSX | GCTGCCTACTTACCCGTCTG | AGGTTTGCCTGCACCACTC |
