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. 2020 Aug 18;7(5):471–482. doi: 10.1093/rb/rbaa025

Figure 4.

Figure 4

HUVEC phenotype 24 h after seeding on collagen scaffolds. Media from HUVEC cultures on non-cross-linked scaffolds (non-XL), EDC/NHS cross-linked scaffolds (XL) with no peptide or functionalized with GFOGER or cell culture plates coated with BSA was sampled after 24 h. (A) LDH activity measured by absorbance at 490 nm. Significance for each condition compared with non-cross-linked scaffolds is shown. Cross-linking prevented scaffold shrinking and degradation in culture media, and reduced cell death after 24 h. Covalent linkage of THPs did not cause additional cytotoxicity. (B) VCAM-1 secretion. Significance for each condition compared with non-cross-linked scaffolds is shown. Scaffold cross-linking and THP functionalization did not result in a dramatic increase in VCAM-1 release, indicating that scaffold preparation does not trigger pro-inflammatory activation.