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. 2020 Aug 18;7(5):471–482. doi: 10.1093/rb/rbaa025

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© The Author(s) 2020. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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HUVEC seeding on top of collagen scaffolds. HUVECs were seeded onto the top surface of EDC/NHS cross-linked scaffolds without peptides or with GFOGER, VWFIII_Nle or GFOGER + VWFIII_Nle, cultured for 1, 4 or 7 days, fixed and stained with Hoechst 33342 (blue) and Rhodamine–Phalloidin (red). (A) Representative Z-stacks from plan views of the top 50 µm of scaffolds. HUVECs formed monolayers lining the inner pore surface after 1 day in all conditions, and after 4 and 7 days on scaffolds functionalized with THPs. (B) Cell number on top of scaffolds. Cell number dropped after 1 day without peptides but did not significantly vary in the presence of GFOGER and GFOGER + VWFIII_Nle. Columns were compared using two-way ANOVA followed by Bonferroni’s multiple comparison tests. Significance for each condition compared with cross-linked scaffolds without peptide on the same day is shown.