Table 2.
Primers used for PCR and real-time PCR analysis of relative gene expression.
| Gene1 type | Name | Accession number | Forward primer (5′) | Reverse primer (3′) | Product Length |
|---|---|---|---|---|---|
| Target | CRT | JN628439.2 | CTCTTCAAAGGTCTGGGCTTGG | CAGAACTCGATGACGGGTGA | 281 |
| Target | AGAT | NM204745.1 | ACATCTTGCACCTGACTACCG | ACAGTGGGTGATCATCAGGAA | 206 |
| Target | GAMT | XM015299974.2 | ACACAAGGTGGTGCCACTGA | CGAGGTGAGGTTGCAGTAGG | 199 |
| Reference | β-actin | NM205518.1 | AATGGCTCCGGTATGTGCAA | GGCCCATACCAACCATCACA | 112 |
| Reference | CycA | GQ849480.1 | GGCTACAAGGGCTCCTGCTT | CCGTTGTGGCGCGTAAA | 77 |
| Reference | RPLP0 | NM204987 | ACACTGGTCTCGGACCTGAGAA | AGCTGCACATCACTCAGAATTTCA | 100 |
1
Abbreviations: AGAT, arginine glycine amidinotransferase; CycA, cycline A; CRT, creatine transporter; GAMT, guanidinoacetate N-methyltransferase; RPLP0, 60S acidic ribosomal protein P0.