Table 2.

Effects of light intensity and flooring type on heterophil-to-lymphocyte (H:L) ratios, serum corticosterone (CORT) concentrations (ng/mL), and IRT eye and beak surface temperatures (°C) of broilers in pens with light intensities of 2, 5, or 10 lx (L) on litter or wire flooring (F) in 2 studies.

Flooring (F) 1		Litter			Wire			P-value2
Light lx (L)		2	5	10	2	5	10	SEM	L	F	L∗F
Age (D)
Study 1
30	H:L	0.26	0.37	0.34	0.24	0.24	0.35	0.20	NS	NS	NS
	CORT	4.98	12.65	10.62	10.84	9.61	13.07	1.79	NS	NS	NS
	Eye temp	33.12	33.31	33.30	33.59	33.04	32.93	0.20	NS	NS	0.08
	Beak temp	33.50	33.44	33.86	34.05	33.71	32.44	0.50	NS	NS	NS
38	H:L	0.16b	0.18b	0.13b	0.28a	0.32a	0.40a	0.12	NS	0.0001	NS
	CORT	5.65	6.82	5.20	5.35	5.55	5.53	1.18	NS	NS	NS
	Eye temp	32.91	33.02	33.44	33.38	33.01	32.60	0.28	NS	NS	0.07
	Beak temp	33.68a	33.31a	33.97a	33.09a	33.97a	29.84b	0.62	0.04	0.003	0.004
Study 2
29	H:L	0.22	0.18	0.24	0.20	0.20	0.19	0.08	NS	NS	NS
	CORT	6.79	6.91	7.31	6.38	6.62	6.72	1.18	NS	NS	NS
	Eye temp	33.83	33.50	33.91	34.28	33.75	33.88	0.23	NS	NS	NS
	Beak temp	33.60	32.94	32.93	34.06	32.53	34.36	0.59	NS	NS	NS
	Core temp	41.19	41.50	41.25	41.50	41.43	41.42	0.10	NS	NS	NS
40	H:L	0.24b	0.18b	0.19b	0.42a	0.29a	0.27a	0.17	NS	0.03	NS
	CORT	6.56	6.67	6.18	7.22	7.94	6.99	1.20	NS	0.06	NS
	Eye temp	34.16a	34.14a	33.92a	34.04a	33.87a	32.84b	0.22	0.004	0.01	0.05
	Beak temp	34.53	34.28	34.12	34.27	34.77	32.89	0.53	NS	NS	NS
	Core temp	41.47	41.49	41.54	41.59	41.39	41.32	0.09	NS	NS	NS

^a,bMeans not sharing the same superscript letter across each row differ at P ≤ 0.05.

Abbreviation: lx, lux.

¹Flooring treatments were litter in all pens until day 29 in study 1and day 28 in study 2 when half of the population from each litter pen (N = 6 pens) were moved to a wire flooring pen (N = 6 pens; 50 birds per pen) with the same light intensity as the corresponding source litter pen.

²ANOVA P-values represent the overall effects, the main effects of light intensity (L) and flooring type (F) and the interaction of light intensity and flooring type (L∗F).