Figure 1.

Effects of in ovo feeding of N-acetyl-L-glutamate (NAG) on relative mRNA expression of nutrient transporters in the jejunum (A) and duodenum (B) of broilers at 7 D post-hatch. The mRNA expression levels were determined by quantitative real-time PCR and calculated relative to the GAPDH gene. Data are presented with means ± SEM (standard error) of 6 replicates. NC = non-punctured control group; SC = saline-injected control group (eggs injected with 300 μL of saline water at 17.5 D of incubation); NAG = NAG solution–injected group (eggs injected with 300 μL of saline water containing NAG 1.5 mg/egg at 17.5 D of incubation); B⁰AT = solute carrier family 6, member 19 (SLC6A19); Pept1 = peptide transporter-1 (SLC15A1); EAAT3 = excitatory amino acid transporter 3 (SLC1A1); rBAT = solute carrier family 3, member1 (SLC3A1).Values within the same gene without common superscript (a-b) differed significantly (P < 0.05).