Table 2.
Effects of in ovo feeding of N-acetyl-L-glutamate (NAG) on embryonic characteristics at 19.5 D of incubation and hatchability of broilers.1
| Variables | Treatment groups |
SEM5 | P-value | ||
|---|---|---|---|---|---|
| NC2 | SC3 | NAG4 | |||
| SEW6(g) | 59.46 | 59.22 | 59.15 | 0.07 | 0.132 |
| 17.5 DOI EW7(g) | 53.87 | 55.96 | 53.67 | 0.53 | 0.149 |
| 19.5 DOI EW8 (g) | 53.21 | 55.57 | 53.03 | 0.59 | 0.150 |
| EBW9 (g) | 43.68 | 45.93 | 43.91 | 0.55 | 0.194 |
| YSW10 (g) | 9.09 | 10.73 | 9.29 | 0.31 | 0.056 |
| YSW/EBW (%) | 20.85 | 23.38 | 21.14 | 0.57 | 0.148 |
| Hatchability (%) | 84.62 | 84.62 | 80.34 | 1.65 | 0.502 |
| Rate of healthy chicks (%) | 97.64 | 94.34 | 92.62 | 1.19 | 0.225 |
| BW11/SEW (%) | 71.20 | 69.83 | 70.17 | 0.28 | 0.162 |
1
N = 6, one embryo from per replicate (39 eggs).
2
NC: non-punctured control group.
3
SC: saline-injected control group (eggs injected with 300 μL of saline water at 17.5 D of incubation).
4
NAG: NAG solution–injected group (eggs injected with 300 μL of saline water containing NAG 1.5 mg/egg at 17.5 D of incubation).
5
SEM: standard error of the mean.
6
SEW: set egg weight.
7
17.5 DOI WE: egg weight at 17.5 DOI.
8
19.5 DOI EW: egg weight at 19.5 DOI.
9
EBW: embryo body weight at 19.5 DOI.
10
YSW: yolk sac weight at 19.5 DOI.
11
BW: average body weight of hatchlings.