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(A) Reverse transcription-polymerase chain reaction analysis of six fragments of the complete NDV genome. (M) 200 bp DNA ladder. (B) Infection of CEF cells by the NDV. CEF cells were infected with 105 EID50 HR09 and NDV/rHR09 viruses. At 36 hpi, the CPE induced by NDV strains was observed on an inverted microscope, the scale bare = 1 mm. Abbreviations: CEF, chicken embryo fibroblast; EID50, 50% egg infectious dose; NDV, Newcastle disease virus.
