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. Author manuscript; available in PMC: 2020 Oct 30.
Published in final edited form as: Placenta. 2018 Jul 31;72-73:1–9. doi: 10.1016/j.placenta.2018.07.014

Figure 3. Quantification of total BODIPY-fatty acid uptake in primary isolated human term trophoblast cells with and without inhibitors.

Figure 3.

Primary human trophoblast were isolated and plated onto 96 well plates. Cells were pre-treated with inhibitors (fetal bovine serum [FBS], Triacsin C [Acyl-CoA inhibitor], CB-2 [FATP2 inhibitor]) 1h before addition of 2μM BODIPY-fatty acid, and total uptake was measured after 20min of incubation with BODIPY-fatty acid using plate reader fluorescence as a marker. Trophoblast tissues were assayed at two time points after initial plating, at 8h to measure cytotrophoblast uptake, or at 72h allow for differentiation to syncytiotrophoblast (SCT) before measuring uptake. (A) BODIPY-C5, (B) BODIPY-C12, and (C) BODIPY-C16 uptake was measured. These data indicate uptake of long and very long-chain fatty acids are transporter dependent. Data are Mean±SEM, n=5 placentas. *= p<0.05, **=p<0.01 vs corresponding cell type control. #=p<0.05 CTB vs SCT.