Table 3.
Detection (+/−) and quantification (log10 cfu/g feces) of CTX-M-1-Escherichia coli in broilers in experiments 2 (dose 102 cfu/mL) and 3, determined at n hours p.i. at days 5 to 7 (8:00 am and 4:00 pm), 8 to 14, 16, 19, and 21 (8:00 am).
| Exp | Iso | Dose | Intervention | Bird ID | Seeder or contact | D5 8:00 | D5 4:00 | D6 8:00 | D6 4:00 | D7 8:00 | D7 4:00 | D8 8:00 | D9 8:00 | D10 8:00 | D11 8:00 | D12 8:00 | D13 8:00 | D14 8:00 | D16 8:00 | D19 8:00 | D21 8:00 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| hours p.i. | 0 | 8 | 24 | 32 | 48 | 56 | 72 | 96 | 120 | 144 | 168 | 192 | 216 | 264 | 336 | 384 | |||||
| 2 | 5 | 102 | None | 205 | Seeder | − | + | 3.63 | + | 5.48 | + | 5.78 | 3.57 | 6.52 | 6.63 | 7.57 | 5.44 | 6.40 | 3.88 | 5.57 | 5.25 |
| 5 | 102 | None | 212 | Seeder | − | − | 2.57 | + | 4.63 | + | 5.57 | 4.52 | 4.48 | 4.63 | 2.57 | 4.57 | 5.36 | 4.52 | 4.63 | 4.70 | |
| 5 | 102 | None | 275 | Seeder | − | − | 3.52 | + | 4.63 | + | 2.57 | 5.78 | 3.63 | 3.52 | 4.57 | 4.30 | 5.05 | 3.52 | 6.78 | 4.30 | |
| 5 | 102 | None | 289 | Seeder | − | − | 2.63 | + | 4.52 | + | 3.48 | 3.44 | 3.78 | 2.48 | 3.00 | 2.44 | 2.52 | 4.70 | 4.78 | 6.36 | |
| 5 | 102 | None | 296 | Seeder | + | − | − | + | 3.63 | + | 3.63 | 5.40 | 6.52 | 3.57 | 4.57 | 5.52 | 3.48 | 6.63 | 4.70 | 5.22 | |
| 5 | 102 | None | 226 | Contact | − | − | − | + | 2.48 | + | 3.57 | 5.88 | 5.70 | 4.63 | 7.88 | 4.36 | 4.27 | 4.57 | 4.48 | 5.12 | |
| 5 | 102 | None | 233 | Contact | − | − | − | − | 2.33 | + | 2.52 | 2.40 | 2.78 | 2.52 | 5.52 | 3.40 | 4.70 | 4.63 | 6.57 | 6.13 | |
| 5 | 102 | None | 240 | Contact | − | − | − | + | 2.70 | + | 3.52 | 2.70 | 2.48 | 2.70 | 3.27 | 2.63 | 2.57 | 3.57 | 4.57 | 4.27 | |
| 5 | 102 | None | 261 | Contact | − | − | 2.63 | − | 2.44 | + | 2.33 | 2.48 | 3.52 | 3.44 | 2.27 | 2.63 | 3.44 | 6.57 | 5.44 | 4.20 | |
| 5 | 102 | None | 282 | Contact | − | + | 2.63 | + | 4.30 | + | 5.57 | 7.44 | 6.48 | 4.70 | 4.78 | 5.10 | 3.63 | 4.78 | 4.44 | 7.52 | |
| 6 | 102 | CEP | 220 | Seeder | − | − | − | − | − | − | 2.88 | 2.48 | 4.57 | 3.70 | 5.57 | 4.78 | 2.52 | 4.63 | 4.57 | 3.63 | |
| 6 | 102 | CEP | 234 | Seeder | − | − | 2.27 | + | 2.40 | + | 3.36 | 4.40 | 5.52 | 5.78 | 3.78 | 3.30 | 3.40 | 5.52 | 4.48 | 5.40 | |
| 6 | 102 | CEP | 241 | Seeder | − | − | − | − | 2.44 | − | 2.48 | 2.40 | 3.70 | 4.63 | 5.63 | 4.48 | 2.57 | 2.63 | 2.52 | 3.40 | |
| 6 | 102 | CEP | 269 | Seeder | − | − | − | − | − | + | 2.44 | 2.57 | 2.63 | 2.52 | 3.63 | 2.70 | 3.44 | 2.48 | 2.57 | 2.48 | |
| 6 | 102 | CEP | 290 | Seeder | − | − | − | − | − | − | 2.44 | 2.36 | 2.57 | 2.48 | 3.52 | 2.52 | 2.63 | 2.48 | 3.63 | 3.33 | |
| 6 | 102 | CEP | 206 | Contact | − | − | − | − | − | − | 2.63 | 3.52 | 4.57 | 4.00 | 5.70 | 2.52 | −2 | ||||
| 6 | 102 | CEP | 227 | Contact | − | − | − | − | − | − | 2.52 | 2.52 | 2.57 | 3.88 | 3.63 | 3.48 | 4.52 | 3.57 | 4.52 | 4.27 | |
| 6 | 102 | CEP | 248 | Contact | − | − | − | − | − | − | 2.70 | 2.57 | 2.48 | 3.70 | 5.63 | 4.78 | 6.57 | 3.57 | 4.48 | 5.70 | |
| 6 | 102 | CEP | 255 | Contact | − | − | − | − | − | − | 2.63 | 2.40 | 3.40 | 3.63 | 2.57 | 3.95 | 3.22 | 3.57 | 3.48 | 3.18 | |
| 6 | 102 | CEP | 276 | Contact | − | + | − | − | − | + | 2.52 | 2.52 | 3.48 | 4.52 | 5.44 | 2.70 | 3.78 | 5.52 | 5.48 | 5.57 | |
| 7 | 102 | CEP | 207 | Seeder | − | − | 2.78 | + | 3.63 | + | 2.57 | 2.52 | 3.57 | 4.63 | 3.70 | 3.63 | 5.88 | 4.78 | 4.57 | 4.33 | |
| 7 | 102 | CEP | 242 | Seeder | − | + | − | − | − | − | − | − | 4.00 | 2.78 | 3.78 | 4.63 | 3.63 | 5.00 | 4.36 | 3.44 | |
| 7 | 102 | CEP | 277 | Seeder | − | − | − | − | − | − | − | − | − | 2.78 | 2.70 | 2.57 | 3.63 | 3.57 | 3.48 | 4.30 | |
| 7 | 102 | CEP | 291 | Seeder | − | − | − | − | − | − | − | − | − | 2.57 | 2.78 | 2.40 | 2.40 | 3.63 | 3.63 | 4.33 | |
| 7 | 102 | CEP | 298 | Seeder | − | − | − | − | − | − | − | − | − | 2.63 | 5.57 | 4.88 | 4.63 | 5.70 | 3.63 | 4.44 | |
| 7 | 102 | CEP | 214 | Contact | − | − | − | − | − | − | − | 3.44 | 3.63 | 4.63 | 4.63 | 4.36 | 3.57 | 3.48 | 4.57 | 4.27 | |
| 7 | 102 | CEP | 235 | Contact | − | − | − | − | − | − | − | 2.48 | 2.78 | 2.88 | 2.88 | 2.48 | 3.57 | 3.52 | 4.48 | 4.44 | |
| 7 | 102 | CEP | 256 | Contact | − | − | − | − | − | − | − | 2.48 | 2.63 | 2.52 | 3.63 | 3.52 | 2.30 | 4.52 | 4.44 | 5.25 | |
| 7 | 102 | CEP | 263 | Contact | − | − | − | − | − | − | − | 2.57 | 2.52 | 2.70 | 4.33 | 3.30 | 3.36 | 3.70 | 2.44 | 2.40 | |
| 7 | 102 | CEP | 284 | Contact | − | − | − | − | − | − | − | − | 2.63 | 3.48 | 3.57 | 5.57 | 2.70 | 3.78 | 3.88 | 3.25 | |
| 3 | 2 | 102 | None | 309 | Seeder | − | − | − | + | 2.30 | + | 2.52 | 2.52 | 4.40 | 4.48 | 3.36 | 4.27 | 4.25 | 3.57 | 3.22 | 5.27 |
| 2 | 102 | None | 333 | Seeder | − | − | − | + | 3.25 | + | 4.57 | 4.48 | 3.57 | 5.44 | 5.25 | 5.40 | 7.25 | 5.48 | 6.03 | 5.06 | |
| 2 | 102 | None | 341 | Seeder | − | − | 3.70 | + | 4.22 | + | 5.48 | 2.48 | 5.57 | 4.33 | 4.27 | 4.36 | 5.57 | 4.52 | 5.18 | 6.05 | |
| 2 | 102 | None | 349 | Seeder | − | − | − | + | 2.70 | + | 3.57 | 3.48 | 5.48 | 4.15 | 6.25 | 5.15 | 3.36 | 4.48 | 5.18 | 5.33 | |
| 2 | 102 | None | 373 | Seeder | − | − | − | − | − | + | 2.36 | 4.52 | 4.15 | 5.48 | 5.30 | 3.57 | 5.20 | 5.52 | 5.25 | 5.33 | |
| 2 | 102 | None | 317 | Contact | − | − | − | − | − | + | 3.57 | 4.40 | 4.70 | 7.30 | 4.33 | 5.52 | 4.63 | 4.63 | 5.12 | 6.06 | |
| 2 | 102 | None | 325 | Contact | − | − | − | − | 2.13 | + | 2.52 | 3.44 | 5.44 | 6.13 | 5.36 | 5.27 | 5.25 | 4.57 | 4.27 | 7.20 | |
| 2 | 102 | None | 357 | Contact | − | − | − | − | − | + | 3.48 | 4.57 | 4.57 | 5.33 | 6.18 | 7.20 | 6.33 | 4.88 | 5.36 | 6.33 | |
| 2 | 102 | None | 365 | Contact | − | − | − | − | − | + | 4.40 | 3.52 | 4.52 | 7.40 | 4.30 | 6.00 | 6.22 | 4.57 | 4.25 | 5.84 | |
| 2 | 102 | None | 381 | Contact | − | − | − | − | − | + | 2.57 | 4.52 | 4.70 | 4.22 | 5.18 | 4.57 | 4.27 | 5.63 | 4.44 | 5.40 | |
| 6 | 102 | SYN | 305 | Seeder | − | − | − | − | − | − | 2.33 | 2.70 | 2.40 | 2.13 | 5.15 | 5.33 | 6.40 | 3.52 | 4.36 | 5.48 | |
| 6 | 102 | SYN | 329 | Seeder | − | − | − | − | − | − | − | − | − | − | − | 2.48 | 2.27 | 2.57 | 3.06 | 5.40 | |
| 6 | 102 | SYN | 337 | Seeder | − | − | − | − | − | − | − | − | − | − | − | − | 2.48 | − | 2.33 | 4.48 | |
| 6 | 102 | SYN | 353 | Seeder | − | − | − | − | − | − | − | − | − | − | 2.01 | 2.12 | 2.36 | 2.52 | 4.20 | 4.57 | |
| 6 | 102 | SYN | 361 | Seeder | − | − | − | − | − | + | 2.36 | − | 2.52 | − | 2.57 | 2.25 | 2.13 | 2.78 | 4.15 | 5.36 | |
| 6 | 102 | SYN | 313 | Contact | − | − | − | − | − | − | − | − | − | 1.85 | 2.15 | 2.40 | 2.15 | 2.70 | 2.15 | 3.36 | |
| 6 | 102 | SYN | 321 | Contact | − | − | − | − | − | − | − | 2.52 | − | − | − | 3.00 | 2.06 | 4.00 | 4.13 | 3.48 | |
| 6 | 102 | SYN | 369 | Contact | − | − | − | − | − | − | − | − | − | − | − | 2.36 | 3.00 | 3.57 | 3.13 | 3.48 | |
| 6 | 102 | SYN | 377 | Contact | − | − | − | − | − | − | − | − | − | − | 2.57 | 2.40 | 3.18 | 2.63 | 2.48 | 4.30 | |
| 6 | 102 | SYN | 385 | Contact | − | − | − | − | − | − | − | − | − | − | 2.48 | 2.33 | 4.57 | 4.70 | 3.33 | 4.63 | |
| 7 | 102 | SYN | 314 | Seeder | − | − | 2.01 | + | 2.40 | + | 4.20 | 2.36 | 3.25 | 4.06 | 4.30 | 3.70 | 4.12 | +1 | 4.25 | 3.33 | |
| 7 | 102 | SYN | 338 | Seeder | − | − | − | + | 4.48 | + | 6.40 | 4.44 | 4.44 | 4.52 | 3.57 | 4.63 | 3.70 | 3.63 | 5.12 | 4.20 | |
| 7 | 102 | SYN | 354 | Seeder | − | − | − | − | − | + | − | 3.57 | 2.30 | 5.18 | 4.12 | 4.36 | 3.44 | 3.63 | 6.27 | 4.52 | |
| 7 | 102 | SYN | 370 | Seeder | − | − | − | − | − | + | 3.27 | 4.57 | 3.48 | 3.27 | 3.12 | 3.36 | 3.40 | − | 6.44 | 3.44 | |
| 7 | 102 | SYN | 394 | Seeder | − | − | − | − | 2.57 | + | 3.48 | 3.63 | 4.20 | 4.13 | 4.33 | 4.25 | 5.88 | 4.88 | 3.03 | 4.25 | |
| 7 | 102 | SYN | 322 | Contact | − | − | − | − | 3.63 | + | 3.48 | 3.57 | 4.30 | 4.30 | 5.52 | 5.30 | 4.52 | 4.78 | 5.20 | 5.52 | |
| 7 | 102 | SYN | 330 | Contact | − | − | − | − | 3.44 | + | 2.27 | 3.57 | 4.44 | 3.70 | 4.20 | 5.44 | 5.25 | 4.48 | 5.36 | 4.30 | |
| 7 | 102 | SYN | 362 | Contact | − | − | − | − | 2.33 | + | 2.48 | 2.78 | 5.05 | 5.22 | 3.33 | 6.27 | 4.70 | 3.70 | 3.90 | 4.15 | |
| 7 | 102 | SYN | 378 | Contact | − | − | − | − | − | + | 3.27 | 3.70 | 2.95 | 4.33 | 5.57 | 5.22 | 6.44 | 5.70 | 6.36 | 6.70 | |
| 7 | 102 | SYN | 386 | Contact | − | − | − | − | − | + | 3.27 | 3.44 | +1 | 5.18 | 4.05 | 4.30 | 4.44 | 3.70 | 4.12 | 5.44 | |
| 8 | 102 | SYN | 315 | Seeder | − | − | 2.52 | + | 4.57 | + | 3.33 | 3.52 | 5.48 | 2.91 | 3.85 | 3.40 | 3.18 | 2.44 | 3.00 | 2.78 | |
| 8 | 102 | SYN | 347 | Seeder | − | − | 2.57 | + | 2.52 | + | 3.44 | 4.25 | 4.57 | 3.33 | +1 | 3.52 | 3.52 | 2.40 | 2.44 | 4.57 | |
| 8 | 102 | SYN | 355 | Seeder | − | − | − | + | 5.36 | + | 5.44 | 6.12 | 4.27 | 3.22 | 4.30 | 3.10 | 4.12 | 2.52 | 3.40 | 3.15 | |
| 8 | 102 | SYN | 363 | Seeder | − | − | − | + | 4.36 | + | 3.48 | 6.40 | 4.88 | 3.70 | 4.63 | 4.48 | 3.63 | 2.78 | 2.44 | 3.52 | |
| 8 | 102 | SYN | 379 | Seeder | − | − | − | + | 4.25 | + | 3.70 | 4.10 | 3.63 | 4.12 | 3.33 | 4.18 | 4.48 | 2.63 | 2.27 | 3.25 | |
| 8 | 102 | SYN | 307 | Contact | − | − | − | − | − | + | 4.00 | 2.15 | 3.40 | 6.27 | 3.33 | 3.08 | 3.44 | 2.70 | +1 | 3.40 | |
| 8 | 102 | SYN | 323 | Contact | − | − | − | − | 2.48 | + | 2.30 | 2.99 | 3.48 | 3.48 | 4.30 | 4.27 | 3.57 | 3.88 | 3.52 | 4.36 | |
| 8 | 102 | SYN | 331 | Contact | − | − | − | − | 2.25 | + | 4.63 | 4.44 | 2.63 | 4.22 | 3.33 | 4.40 | 4.48 | − | 3.20 | 4.52 | |
| 8 | 102 | SYN | 387 | Contact | − | − | − | − | 2.40 | + | 2.78 | 3.63 | 4.44 | 4.27 | 3.36 | 3.27 | 2.52 | 2.70 | 3.13 | 5.57 | |
| 8 | 102 | SYN | 395 | Contact | − | − | − | − | 2.18 | + | 3.48 | 4.36 | 4.40 | 4.36 | 3.40 | 4.20 | 4.44 | 3.52 | 3.06 | 3.70 | |
Abbreviations: CEP, competitive exclusion product; Exp, experiment; Iso, isolator; p.i., post inoculation; SYN, selection of pre- and probiotics.
1
+ in quantification series are broilers excreting CTX-M-1-E. coli (i.e., growth of E. coli on MacConkey + cefotaxime), but excretion values were missing.
2
Chick died.