Table 2.

Plasma Ig concentrations of laying hens at 22, 26, 30, 34, and 38 WOA exposed to different ammonia concentrations under 20°C and 35°C.

Item1	IgG (μg/mL)	IgM (μg/mL)	IgA (μg/mL)
T (°C)
20	1,467.53 ± 25.32a	465.74 ± 4.91	217.18 ± 2.64a
35	1,522.54 ± 27.14b	476.44 ± 5.00	202.31 ± 3.27b
AC (ppm)
≤5	1,350.32 ± 20.48c	469.71 ± 5.31	224.09 ± 3.52a
20	1,512.02 ± 29.85b	468.58 ± 5.64	201.68 ± 3.29b
45	1,622.76 ± 38.75a	474.97 ± 7.17	203.47 ± 3.94b
WOA
22	1,274.80 ± 23.54c	483.02 ± 7.98	216.74 ± 3.90a
26	1,308.15 ± 23.99c	481.26 ± 7.53	211.66 ± 4.50a,b
30	1,342.49 ± 23.80c	469.55 ± 8.44	198.53 ± 4.00b
34	1,674.38 ± 30.10b	465.32 ± 7.24	204.68 ± 5.47a,b
38	1,875.34 ± 46.28a	456.29 ± 7.78	217.13 ± 5.50a
P-value2
T	<0.05	NS	<0.05
AC	<0.05	NS	<0.05
WOA	<0.05	NS	<0.05
T × AC	NS	<0.05	<0.05
T × WOA	NS	NS	<0.05
AC × WOA	<0.05	<0.05	<0.05

^a–cMeans within main effects without a common letter differ (P < 0.05).

¹T, temperature; AC, ammonia concentration; WOA, week of age.

²NS, no significance.