Effects of supplementation with sweeteners on the intestinal integrity and permeability of broiler chickens. (A) Villus height of the jejunum. (B) Crypt depth of the jejunum. (C) Villus height/crypt depth of the jejunum. (D) Activity of serum diamine oxidase (DAO). Data are presented as mean value ± SEM (n = 6). Values without the same letter (a, b) represent statistically significant differences (P < 0.05). Abbreviations: CON, broiler chickens fed a basal diet; STE, broiler chickens fed a basal diet supplemented with 250 mg/kg stevioside; SUC, broiler chickens fed a basal diet supplemented with 100 mg/kg sucralose; SAC, broiler chickens fed a basal diet supplemented with 600 mg/kg saccharin sodium.