Table 1.
Rhythms in locomotor activity and sleep behavior in Cntnap2 KO mice were disrupted by dim light at night (DLaN).
| Cntnap2 KO | Cntnap2 KO + DLaN | WT | WT + DLaN | Genotype | Treatment | |
|---|---|---|---|---|---|---|
| Locomotor Activity rhythms | ||||||
| Power (% variance) | 29.5 ± 2.1 | 21.6 ± 1.8* | 39.2 ± 2.1 | 26.6 ± 1.9* | F = 13.789; P < 0.001 | F = 26.780; P < 0.001 |
| Cage Activity (a.u./hr) | 139.2 ± 11.6 | 84.1 ± 9.9* | 172.6 ± 11.6 | 93.1 ± 10.9* | F = 3.716; P = 0.063 | F = 37.428; P < 0.001 |
| Activity in day (%) | 20.8 ± 1.9 | 26.1 ± 1.8* | 15.9 ± 1.0 | 23.1 ± 1.1* | F = 6.097; P = 0.019 | F = 15.346; P < 0.001 |
| Onset Variability (min) | 25.7 ± 2.1 | 48.8 ± 6.2* | 7.1 ± 2.1 | 29.4 ± 6.5* | F = 29.686; P < 0.001 | F = 41.644; P < 0.001 |
| Fragmentation | 9.8 ± 0.9 | 11.6 ± 0.9 | 9.0 ± 0.9 | 11.3 ± 0.9 | F = 1.173; P = 0.287 | F = 2.888; P = 0.089 |
| Sleep Behavior rhythms | ||||||
| Daily sleep (min) | 908.6 ± 43.8 | 1042.6 ± 49.4* | 943.5 ± 52.8 | 1018.0 ± 54.7* | F = 0.011; P = 0.914 | F = 4.762; P = 0.036 |
| Sleep in day (min) | 590.9 ± 17.6 | 564.6 ± 33.1 | 622.0 ± 27.8 | 569.4 ± 50.3 | F = 0.274; P = 0.604 | F = 1.318; P = 0.258 |
| Sleep in night (min) | 317.7 ± 28.2 | 478.0 ± 49.8* | 321.5 ± 32.3 | 448.6 ± 53.2* | F = 0.091; P = 0.764 | F = 11.533; P = 0.002 |
| Bouts in day (#) | 3.7 ± 0.5 | 3.9 ± 0.2 | 4.3 ± 0.2 | 3.3 ± 0.5 | F = 1.118; P = 0.299 | F = 14.492; P < 0.001 |
| Bouts in night (#) | 6.3 ± 0.4 | 5.7 ± 0.4 | 5.8 ± 0.6 | 6.1 ± 0.5 | F = 0.067; P = 0.797 | F = 1.966; P = 0.172 |
| Bout duration day | 177.4 ± 26.7 | 207.5 ± 33.0 | 229.0 ± 54.7 | 161.2 ± 27.1 | F = 1.581; P = 0.217 | F = 1.329; P = 0.257 |
| Bout duration night | 47.7 ± 4.8 | 89.8 ± 15.8* | 54.4 ± 3.9 | 82.7 ± 9.2* | F = 0.007; P = 0.979 | F = 14.676; P < 0.001 |
| Sleep onset (ZT) | 21.2 ± 2.4 | 22.2 ± 0.6* | 24.4 ± 0.2 | 23.2 ± 0.2* | F = 5.639; P = 0.023 | F = 20.617; P < 0.001 |
Comparisons of age-matched WT and Cntnap2 KO mice under LD or DLaN regimen (n = 8/group). Values are shown as averages ± SEM. Data were analyzed with a two-way ANOVA using genotype and treatment as factors. The Holm-Sidak test for multiple comparisons was used when appropriate.
*
indicates significant differences between LD controls and those exposed to DLaN.
P values < 0.05 were considered significant and are shown in bold.