Table 1.
Primers for PCR amplifications to detect MDV, REV, and ALV and for amplifying the entire proviral genome of ALV-J.
| Primer1 | Sequence (5′-3′) | Position (nt)2 | Size of PCR product (bp) |
|---|---|---|---|
| MDV meq F | CCGTCTAGAAGGCGGGCACGGTAC | 133,351-133,374 | 1,113 |
| MDV meq R | CGGAAGCTTAAACATGGGGCATAGACG | 134,437-134,463 | |
| MDV 132F | TGCGATGAAAGTGCTATGGAGG | 127,553-127,574 | 185, 317 449, 581 |
| MDV 132 R | GAGAATCCCTATGAGAAAGCGC | 127,848-127,869 | |
| REV LTR F | CATACTGGAGCCAATGGT | 335-351 | 291 |
| REV LTR R | AATGTTGTAGCGAAGTACT | 609-627 | |
| ALV-J-F | GGATGAGGTGACTAAGAAAG | 5,258-5,277 | 545 |
| ALV-J-R | CGAACCAAAGGTAACACACG | 5,783-5,802 | |
| ALV-A-F | GGATGAGGTGACTAAGAAAG | 5,026-5,045 | 692 |
| ALV-A-R | AGAGAAAGAGGGGTGTCTAAGGAG | 5,694-5,717 | |
| ALV-B-F | GGATGAGGTGACTAAGAAAG | 5,053-5,072 | 847 |
| ALV-B-R | ATGGACCAATTCTGACTCATT | 5,879-5,899 | |
| ALV-C-F | GGATGAGGTGACTAAGAAAG | 5,267-5,286 | 860 |
| ALV-C-R | GAGGCCAGTACCTCCCACG | 6,108-6,126 | |
| ALV-D-F | GGATGAGGTGACTAAGAAAG | 5,020-5,039 | 797 |
| ALV-D-R | ATCCATACGCACCACAGTATTCG | 5,795-5,817 | |
| ALV-K-F | GATGAGGCGAGCCCTCTCTTTG | 5,273-5,294 | 2,354 |
| ALV-K-R | TGTTGGGAGGTAAAATGGCGT | 7,607-7,627 | |
| J-1F | TGTAGTGTTATGCAATACTCTTATGTAACGATGAAAC | 1-37 | 3,621 |
| J-1R | GGCCATTTTCATGTCTAGATT | 3,600-3,620 | |
| J-2F | GGCGAGGGAATGGAATCT | 3,587-3,604 | 3,069 |
| J-2R | CATCGATTTCTTACTCCTGGCGC | 6,633-6,655 | |
| J-3F | CGAGCAGCCATCGATTTCTTACTC | 6,625-6,648 | 1,216 |
| J-3R | CCTGACGACTACGAGCACCTGCATGAAGCGGATGGCTTCA | 7,802- 7,841 |
Abbreviations: ALV, avian leukosis virus.
1
F and R denote the forward and reverse primers for a specific fragment, respectively.