Fig. 4.

In general, as G′ decreases, the product may exhibit more cohesive properties (higher drop weight). Rheologic measurements were performed in a sequence that included a relaxation time of 30 minutes, a frequency sweep from 10 to 0.1 Hz at 0.1 percent strain, followed by an amplitude sweep from 0.1 to 10,000 percent (0.001 to 100) strain at 1 Hz. The gap was 1 mm using a PP25 measuring system at 25°C. Cohesion was measured as drop weight of the samples. Gel was extruded at a constant speed (7.5 mm/minute) from an 18-gauge cannula. Once a constant force was achieved, at least 10 fragments (drops) were collected, and average drop weight (in milligrams) was calculated.