. 2020 Jun 16;99(8):3877–3884. doi: 10.1016/j.psj.2020.04.030

Table 2.

Serum 25-hydroxycholecalciferol (25OHD₃) concentrations of embryos at 462 h of incubation (hoi) after in ovo injection of a 50 μl solution volume at 432 hoi.

Treatment	Serum 25OHD₃ concentration (ng/mL)
Noninjected	8.61^b
Diluent¹	9.74^b
Vitamin D₃²
0.6	8.83^b
1.2	11.24^a
2.4	11.60^a
25OHD₃³
0.6	10.95^a
1.2	12.10^a
2.4	12.29^a
Vitamin D₃ + 25OHD₃⁴
1.2	11.41^a
2.4	10.96^a
4.8	12.20^a
Source of variation
Pooled SEM	0.301
P-value	0.001

^a,bMeans within control, vitamin D₃, 25OHD₃, and vitamin D₃ + 25OHD₃ categories with no common superscript differ significantly (P < 0.05).

Eggs injected with 50 μl commercial MD diluent at 432 hoi.

Eggs injected with 50 μl commercial MD diluent containing vitamin D₃ at 0.6, 1.2, and 2.4 μg at 432 hoi.

Eggs injected with 50 μl commercial MD diluent containing 25OHD₃ at 0.6, 1.2, and 2.4 μg at 432 hoi.

⁴

Eggs injected with 50 μl commercial MD diluent containing a combination of D₃ and 25OHD₃ at 1.2, 2.4, and 4.8 μg at 432 hoi.