Table 3.

Contrast analyses for serum 25-hydroxycholecalciferol (25OHD₃) concentrations at 462 h of incubation (hoi), postinjection hatchability of live embryonated eggs at 492 and 516 hoi, and hatchling somatic variables. Contrasts included vitamin D₃ (D₃), 25OHD_3, and their combination across injection and dosages (0.6, 1.2, 2.4, and 4.8 μg across vitamin D₃ types).

	Serum 25OHD31	H-4922	H-5163	BW4	YFBW5	RLW6	RYW7	PYM8	PYDM9
Contrasts of vitamin D3 types across dosage
D3 vs. 25OHD3	∗∗∗	ns	ns	ns	ns	ns	ns	∗∗	∗∗
D3 vs. D3 + 25OHD3	∗∗∗	†	ns	ns	ns	ns	ns	ns	ns
25OHD3 vs. D3 + 25OHD3	ns	ns	ns	ns	ns	ns	ns	ns	ns
Contrasts of dosages across vitamin D3 types
0.6 vs. 1.2	∗∗∗	ns	ns	ns	ns	ns	ns	ns	ns
0.6 vs. 2.4	∗∗∗	∗	ns	ns	ns	ns	ns	ns	ns
0.6 vs. 4.8	∗∗∗	∗	ns	ns	ns	ns	ns	†	†
1.2 vs. 2.4	ns	ns	ns	ns	ns	ns	ns	ns	ns
1.2 vs. 4.8	ns	ns	ns	ns	ns	†	ns	∗	∗
2.4 vs. 4.8	ns	ns	ns	ns	ns	∗∗	ns	ns	ns

^†Treatment means for the same variable with no common superscript differ significantly (P ≤ 0.1).

^∗Treatment means for the same variable with no common superscript differ significantly (P ≤ 0.05).

^∗∗Treatment means for the same variable with no common superscript differ significantly (P ≤ 0.01).

^∗∗∗Treatment means for the same variable with no common superscript differ significantly (P ≤ 0.005).

Abbreviation: ns = not significant.

¹Serum concentration of 25OHD₃ was determined at 462 hoi.

²Hatchability of live embryonated eggs at 492 hoi.

³Hatchability of live embryonated eggs at 516 hoi.

⁴Hatching BW at 516 hoi.

⁵Yolk-free BW at 516 hoi.

⁶Relative liver weight at 516 hoi.

⁷Relative yolk sac weight at 516 hoi.

⁸Percentage of yolk moisture at 516 hoi.

⁹Percentage of yolk dry matter at 516 hoi.