Table 5.

Effects of in ovo injection treatment (noninjected, diluent injected, and injected with diluent containing vitamin D₃ [D₃] or 25-hydroxycholecalciferol [25OHD₃] or the combination of D₃ + 25OHD₃ [50 μL]) and their dosages, on hatch residue analysis variables (late dead embryo, pipped dead and live embryo, and dead chick at 516 h of incubation (hoi).

	Late dead1	Pipped dead2	Pipped live3	Dead chick4
	-------------------- (%) -----------------------------
Treatment
Noninjected	3.44	0.34	0	0
Diluent5	3.83	1.35	0	1.02
D36
0.6	3.90	0.35	0.36	0.37
1.2	5.40	0.34	0	0.38
2.4	2.16	1.80	1.03	0.34
25OHD37
0.6	4.17	0.74	0.66	1.03
1.2	5.48	0.35	0.36	0.36
2.4	1.71	1.03	0.68	1.03
D3 + 25OHD38
1.2	4.48	0	0	0
2.4	6.48	0.69	0	0.35
4.8	3.17	1.06	0	1.39
Source of variation
Pooled SEM	0.099	0.529	0.149	0.424
P-value	1.114	0.404	0.322	0.473

¹Dead embryos that had not externally pipped at 516 hoi.

²External pipped dead (chick pipped shell and was dead) at 516 hoi.

³External pipped alive (chick pipped shell and was alive) at 516 hoi.

⁴Dead chicks that were found at 516 hoi.

⁵Eggs injected with 50 μl commercial diluent at 432 hoi.

⁶Eggs injected with 50 μl commercial diluent containing vitamin D3 at 0.6, 1.2, and 2.4 μg/egg at 432 hoi.

⁷Eggs injected with 50 μl commercial diluent containing 25OHD3 at 0.6, 1.2, and 2.4 μg/egg at 432 hoi.

⁸Eggs injected with 50 μl commercial diluent containing a combination of D3 and 25OHD3 at 1.2, 2.4, and 4.8 μg/egg at 432 hoi.