Table 1.
Primers used for detection of the follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) gene by reverse transcription polymerase chain reaction.
| Gene | Primer sequences | Amplicon size (bp) | Annealing temperature (°C) | Accession number |
|---|---|---|---|---|
| β-actin-F1 | ACGTCGCACTGGATTTCGAG | 282 | 58 | NM_205518.1 |
| β-actin-R2 | TGTCAGCAATGCCAGGGTAC | |||
| FSHR-F3 | AAGAGCGAGGTCTACATACA | 414 | 52 | XM_025148544.1 |
| FSHR-R4 | GTGGTGTTCCCAGTGATAG | |||
| LHR-F5 | GCTGCTCATTGCTTCGG | 713 | 54 | U92082.1 |
| LHR-R6 | GCTCTGCTCGGCTCTTAC |
1,2
Refers to the forward primer and reverse primer of beta-actin (β-actin, a housekeeping gene used as a control for normalization).
3,4
Indicates the forward primer and reverse primer of the FSHR.
5,6
Indicates the forward primer and reverse primer of the LHR.