Table 2.
Primers used for detection of proliferating cell nuclear antigen (PCNA), steroidogenic acute regulatory protein (StAR), and cytochrome P450 family 11 subfamily A member 1 (CYP11A1) gene by real-time quantitative polymerase chain reaction.
| Gene | Primer sequences | Amplicon size (bp) | Annealing temperature (°C) | Accession number |
|---|---|---|---|---|
| GAPDH-F1 | ACGTCGCACTGGATTTCGAG | 82 | 60 | NM_204305 |
| GAPDH-R2 | TGTCAGCAATGCCAGGGTAC | |||
| PCNA-F3 | GCAGATGTTCCTCTCGTTGTGGAG | 95 | 60 | NM_204170.2 |
| PCNA-R4 | GAGCCTTCCTGCTGGTCTTCAATC | |||
| StAR-F5 | CGCTGCCATCTCCTACCAACAC | 197 | 60 | NM_204686.2 |
| StAR-R6 | AGGACATCTCCATCTCGCTGAAGG | |||
| CYP11A1-F7 | CCGCCACCTCAACACCAAGAC | 157 | 60 | NM_001001756.1 |
| CYP11A1-R8 | CACAAGGAGGCTGAAGAGGATGC |
1,2
Refers to the forward primer and reverse primer glyceraldehyde phosphate dehydrogenase (GAPDH; a housekeeping gene used as a control for normalization).
3,4
Indicates the forward primer and reverse primer of PCNA.
5,6
Indicates the forward primer and reverse primer of StAR.
7,8
Indicates the forward primer and reverse primer of CYP11A1.