Table 1.

Sequences of primers used for quantitative reverse transcription-polymerase chain reaction for detection of chicken and IBDV genes of interest.

Gene name	Primer sequence	Annealing temperature (°C)	Amplicon size (bp)	Accesion number or reference
β-actin	F: 5′-CAACACAGTGCTGTCTGGTGGTA-3′ R: 5′-ATCGTACTCCTGCTTGCTGATCC-3′	61	205	X00,182
IBDV-VP2	F: 5′-CTGACTACCGGCATCGACA-3′ R: 5′-CCACTTGCCGACCATGA-3′	60	149	AF498631
VDR	F: 5′-AGAAGCAAATTCAGCAGCAGGA-3′ R: 5′-AAGGCATCGGAGCCAAAGAC-3′	60	101	NM205098.1
TLR-3	F: 5′-TGCATAAGAAGGAGCAGGAAG-3′ R: 5′-CTGGCCAGTTCAAGATGCAG-3′	60	263	NM001011691
TLR-21	F: 5′-TCAGCTACACCAAAATGTTCAACC-3′ R: 5′-CGTGATTTTGCCTGTGAGC-3′	60	249	NM204278
MDA5	F: 5′-GCAAAACCAGCACTGAATGGG-3′ R: 5′-CGTAAATGCTGTTCCACTAACGG-3′	59	178	(Alkie et al., 2015)
TRIF	F: 5′-GCTGACCAAGAACTTCCTGTGC-3′ R: 5′-AGAGTTCTCATCCAAGGCCACC-3′	60	120	NM001081506.1
MyD88	F: 5′-AGAAGGTGTCGGAGGATGGTG-3′ R: 5′-GGGCTCCAAATGCTGACTGC-3′	57	365	NM001030962
IRF7	F: 5′-CTCCCCTCCTCCAAAAGCTG-3′ R: 5′-CTGGGAGCGAAGGAGGAATG-3′	60	127	(Alkie et al., 2015)
INF-α	F: 5′-ATCCTGCTGCTCACGCTCCTTCT-3′ R: 5′-GGTGTTGCTGGTGTCCAGGATG-3′	60	197	(Alkie et al., 2015)
INF-β	F: 5′-GCCTCCAGCTCCTTCAGAATACG-3′ R: 5′-CTGGATCTGGTTGAGGAGGCTGT-3′	60	223	(Alkie et al., 2015)
OAS	F: 5′-AGAACTGCAGAAGAACTTTGTC-3′ R: 5′-GCTTCAACATCTCCTTGTACC-3′	58	91	(Alkie et al., 2015)
PKR	F: 5′-GGAGGCGGGAATGGAGAAAA-3′ R: 5′-GAGCACATCCGCAGGTAGAG-3′	60	144	(Alkie et al., 2015)
Viperin	F: 5′-GGAGGCGGGAATGGAGAAAA-3′ R: 5′-CAGCTGGCCTACAAATTCGC-3′	58	78	(Alkie et al., 2015)
IL-1β	F: 5′-AACCCGACCAGGTCAACA-3′ R: 5′-CGGTACATACGAGATGGAAAC-3′	61	101	AJ245728.1
IL-12	F: 5′-TCTGCTAAGACCCACGAGA-3′ R: 5′-TTGACCGTATCATTTGCCCAT-3′	60	82	NM213571
IL-6	F: 5′-CAGGACGAGATGTGCAAGAA-3′ R: 5′-TAGCACAGAGACTCGACGTT-3′	59	232	AJ309540

Abbreviations: IBDV, infectious bursal disease virus; IL, interleukin; INF, interferon; MDA-5, melanoma differentiation-associated protein 5.