Table 2.
List of primers used for qPCR.
| Gene symbol1 | Accession number | Forward primer | Reverse primer |
|---|---|---|---|
| GAPDH2 | NM_204305.1 | CCTCTCTGGCAAAGTCCAAG | GGTCACGCTCCTGGAAGATA |
| Beta-actin2 | NM_205518.1 | CAACACAGTGCTGTCTGGTGGTA | ATCGTACTCCTGCTTGCTGATCC |
| HMBS2 | XM_004947916.3 | GGCTGGGAGAATCGCATAGG | TCCTGCAGGGCAGATACCAT |
| CLDN13 | NM_001013611.2 | TGGAGGATGACCAGGTGAAGA | CGAGCCACTCTGTTGCCATA |
| OCLN3 | XM_025144248.1 | ACGGCAGCACCTACCTCAA | GGCGAAGAAGCAGATGAG |
| ZO23 | XM_025144669.1 | GGCAAATCATTGAGCAGGA | ATTGATGGTGGCTGTAAAGAG |
| JAM23 | XM_025149444.1 | AGCCTCAAATGGGATTGGATT | CATCAACTTGCATTCGCTTCA |
1
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HMBS, hydroxymethylbilane synthase; CLDN1, claudin 1; OCLN, occludin; ZO2, tight junction protein 2; JAM2, junctional adhesion molecule 2.
2
Housekeeping gene.
3
Tight junction proteins.