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Western blotting of p-ERK1/2 and p-p38. HD11 cells were induced by AvBD8 (100 ng/mL) for 0, 15, 30, 60, 90, and 180 min. Total cell lysates were analyzed by western blotting using antibodies against p-ERK1/2, p-p38, and GAPDH. Abbreviations: AvBD8, avian β-defensin 8; ERK1/2, extracellular regulated kinases 1/2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
