Table 2.
Specific gene primers used for real-time quantitative PCR.
| Gene | Accession no. | Primers sequences |
|---|---|---|
| HSP70 | NM_001006685.1 | F:GGGGCACTTTTGATGTGT |
| R:CCAGCAATGTCACGCTT | ||
| Nrf2 | NM_205117.1 | F:GGCCTTGTCCTTTGATGA |
| R:GGGTGGCTGAGTTTGATTA | ||
| HO-1 | NM_205344.1 | F:GGCAAGAAGCATCCAGAG |
| R:GTGAAGAAAGCCAACCCTT | ||
| NQO1 | NM_001277619.1 | F:GCTGGGAAGTCACCATCTC |
| R:GACAAAGCACTCGGGGTT | ||
| Prx1 | NM_001277724.2 | F:CTGTCACCTTGCCTGGAT |
| R:ATAGTGCCCAAACCACCTT | ||
| Prx3 | XM_426543.5 | F:GGATTTCACCTTTGTGTGC |
| R:TGGAACTCATTCGCTTTGT | ||
| CAT | NM_001031215.2 | F:TTACGGAGGTAGAACAGATGG |
| R:TGTCAGGATACGCAAAGAGA | ||
| SOD1 | NM_205064.1 | F:AGGGAGGAGTGGCAGAAG |
| R:GCAGTGTGGTCCGGTAAG | ||
| SOD2 | NM_204211.1 | F:GGGGAGCCTAAAGGAGAA |
| R:AAGTTTGCGAAGGAACCA | ||
| GCLC | XM_419910.5 | F:ATGATGGAGAAGCAGCAAA |
| R:GCCTGGAATGTTACCTGAA | ||
| GCLM | NM_001007953.1 | F:TGTGCTGCTGCTTAACCATTCCG |
| R:GCAACGCGCACATTGTTCTAGC | ||
| GST | NM_001001777.1 | F:CGTCCAACCAGCAGATAAA |
| R:TCCGTGGTCCTTCAAAAC | ||
| BCL-2 | NM_205339.2 | F:GAGTTCGGCGGCGTGATGTG |
| R:TTCAGGTACTCGGTCATCCAGGTG | ||
| BAK1 | NM_001030920.1 | F:CAGCAGCGAGGCAGCAGAAC |
| R:AACAGCACAGAGCGGACACAAC | ||
| Caspase3 | NM_204725.1 | F:AACACGCCAGGAAACTTG |
| R:TATTCTGCCACTCTGCGAT | ||
| Caspase9 | XM_424580.6 | F:AACTTGCCGACGTTCCA |
| R:GGCTCGTCCTCATTCCC | ||
| β-actin | NM_205518.1 | F:CTCTATCCTGGCCTCCCT |
| R:GGGTGTGGGTGTTGGTAA |
Abbreviations: BAK1, BCL2 antagonist/killer 1; BCL-2, BCL2, apoptosis regulator; Caspase3, caspase 3; Caspase9, caspase 9; CAT, catalase; GCLc, glutamate-cysteine ligase catalytic subunit; GCLm, glutamate-cysteine ligase modifier subunit; GST, glutathione S-transferase alpha 3; HO-1, heme oxygenase 1; HSP70, heat shock 70 kDa protein 2; Nrf2, nuclear factor, erythroid 2 like 2; NQO1, NAD(P)H quinone dehydrogenase 1; Prx1, peroxiredoxin 1; Prx3, peroxiredoxin 3; SOD1, superoxide dismutase 1; SOD2, superoxide dismutase 2.