Table 6.

Interaction between coccidial vaccination, coccidial challenge, and bird age (day) on blood glucose (Glu), total carbon dioxide (TCO₂), hematocrit (HCT), TBARS (malondialdehyde [MDA]), heterophil (HET), and lymphocyte (LYM) levels and the HET:LYM ratio of broiler chickens from day 6 to day 34¹.

Treatment	Vaccine	Challenge	Age	vGlu, mg/dl	iGlu, mg/dl	TCO2, mmol/l	HCT, %	MDA, μM	HET	LYM	HET:LYM
Main effects
	−			222.4	223.2	22.1	19.0	1.00	2,249.2	5,008.4	0.44
	+			217.6	215.2	22.1	18.4	0.99	2,412.1	5,522.3	0.45
	P <			NS	NS	NS	NS	NS	NS	NS	NS
		−		221.7	222.4	21.9	18.9	0.99	2,432.0	5,445.0	0.44
		+		218.4	216.0	22.3	18.5	0.99	2,229.3	5,085.8	0.45
		P <		NS	NS	NS	NS	NS	NS	NS	NS
			6	215.6	212.4	20.7	15.1	1.47	417.7	1,997.2	0.24
			8	201.2	202.6	20.3	16.5	1.14	583.7	2,161.3	0.25
			13	223.2	227.6	22.1	18.9	1.22	4,461.1	4,580.3	0.99
			20	218.8	217.4	21.5	19.6	0.94	2,436.2	8,541.5	0.30
			27	233.6	230.2	24.1	21.7	0.78	2,355.0	8,106.6	0.30
			34	227.8	225.0	24.0	20.5	0.42	3,730.3	6,205.4	0.60
			P <	0.05	0.05	0.001	0.001	0.001	0.001	0.001	0.001
			L <	0.05	0.05	0.001	0.001	0.001	0.01	0.001	0.01
			Q <	0.05	0.05	0.001	0.001	0.001	0.001	0.001	0.001
SEM				12.94	13.83	1.26	1.33	0.166	668.73	722.50	0.102
Interaction terms
Vaccine × challenge				NS	NS	NS	NS	NS	NS	NS	NS
Vaccine × age				NS	NS	NS	NS	NS	NS	NS	NS
Challenge × age				NS	NS	0.05	NS	NS	NS	NS	NS
Vaccine × challenge∗age				NS	NS	NS	NS	NS	NS	NS	NS

Superscripts for column headers refer to either VetScan ‘v’ or iStat ‘i’ devices that were used.

¹L: linear P value; Q: quadratic P value; NS: not statistically significant (P > 0.05).