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. 2020 Jun 20;99(9):4586–4597. doi: 10.1016/j.psj.2020.05.025

Table 1.

Primers used for real-time PCR analysis.

Genes Primer sequences Size of PCR product (bp) Accession no.
P450scc F: 5′-ACGCTGGTGCAGGTTGGTCTC-3′ 154 XM_015872775.1
R: 5′-GCTCAGTCCCTTGAAGTGCTT-3′
3β-HSD F: 5′-CTGGGGAAACAGCAACAGCAG-3′ 175 XM_015874122.1
R: 5′-TTATTTTGGTTCTGGGGATGA-3′
P450arom F: 5′-GAATTCTTCCCAAAACCGAATGAG-3′ 162 XM_015872888.1
R: 5′-GCACCGTCTCAGAAGAGTCACCAG-3′
17β-HSD F: 5′-TCTTGGTGTGGGAATGTCAA-3′ 187 XM_015849994.1
R: 5′-CCGGAATAGAAGGAACACCA-3′
ESR1 F: 5′-GCCTGGCAGGATTTCACTCT-3′ 154 XM_017010382.2
R: 5′-GCTTCCCTCATCCCAAAGCT-3′
FSHR F: 5′-CGTTCATGGGCCTGAGTTCT-3′ 179 XM_015856889.1
R: 5′-CAAAACAACAGGCCCGATGG-3′
LHR F: 5′-CAGTCAACTCCTGCGCAAAC-3′ 195 NM_137366.4
R: 5′-CCTGTGAGGCTTTACTGGGG-3′
IL-1β F: 5′-ACCCGCTTCATCTTCTACCG-3′ 173 AB559570.2
R: 5′-TAGCTTGTAGGTGGCGATGT-3′
IL-6 F: 5′-GGTGATAAATCCCGATGAAGT-3′ 142 XM_015853677.1
R: 5′-TCTCCATAAACGAAGTAAAGTCTC-3′
NF-κΒ F: 5′-TCAACGCAGGACCTAAAGACAT-3′ 165 NM_001279309.1
R: 5′-GCAGATAGCCAAGTTCAGGATG-3′
TLR4 F: 5′-ATGTCCTCTTGCCATCCCAA-3′ 192 XM_015878841.1
R: 5′-TCTCCCCTTTCTGCAGAGTG-3′
IL-18 F: 5′-AGCGTCCAGGTAGAAGATAA-3′ 187 XM_015883845.1
R: 5′-AATATGATGTTACTTTCACCAGGA-3′
IL-12β F: 5′-TGTCTCACCTGCTATTTGCCTTAC-3′ 158 XM_015876070.1
R: 5′-CATACACATTCTCTCTAAGTTTCCACTGT-3′
Caspase3 F: 5′-TGGCGATGAAGGACTCTTCT-3′ 197 XM_017312543.1
R: 5′-CTGGTCCACTGTCTGCTTCA-3′
AGPAT5 F: 5′-TGCTGTTTCTAGGTGGCCTG-3′ 184 XM_015859300.1
R: 5′-ACCCACAGGCAGCCAATTAA-3′
GAPDH F: 5′-TGATGCTCCCATGTTCGTGA-3′ 149 XM_015873412.1
R: 5′-TAAGACCCTCCACGATGCC-3′
β-actin F: 5′-TGAACCCCAAAGCCAACAG-3′ 201 NM_001124235.1
R: 5′-CCACAGGACTCCATACCCAAG-3′