Figure 6. Time-based Scaffold analysis of volunteer H1N1 challenge.

Pooled cells from all volunteers and all time points (381 mass cytometry samples) were binned into 1 of 200 nonoverlapping clusters based on the similarity of their expression across all surface markers. Clusters are displayed as nodes of a force-directed Scaffold graph with the length of edges inversely proportional to the similarity of clusters to each other and to landmark nodes defined by manual gating (Supplemental Figure 5). The colored wedges in cluster nodes indicate the degree of increase (red) or decrease (blue) in the mean relative abundance of a given cell cluster for virus shedders on study days 2–8. n = 35 volunteers.