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. 2020 Oct 12;130(11):6064–6079. doi: 10.1172/JCI136457

Figure 1. BIN2 is highly expressed in human and mouse platelets.

Figure 1

(A) Western blot analysis of BIN2 expression in human tissue lysates; GAPDH was used as loading control. (B) IP of BIN2 was performed with human platelet lysates using an anti-BIN2 antibody. BIN2, STIM1, and RhoA were detected by Western blot. Representative result of 3 independent experiments. Lane 4 was run on the same gel as 1 to 3, but the lanes were noncontiguous. (C) Recombinant GST-tagged STIM1 C-tail incubated with recombinant BIN2 protein. Pulldown of the GST-tagged STIM1 was performed, and the resulting fractions were analyzed by SDS page and silver staining. (D) Western blot analysis of BIN2 expression in the indicated tissue lysates of WT and Bin2–/– mice with GAPDH as loading control. See complete unedited blots in the supplemental material.