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. 2020 Oct 12;130(11):6064–6079. doi: 10.1172/JCI136457

Figure 5. Defective integrin activation and aggregation of Bin2fl/fl,Pf4Cre platelets upon (hem)ITAM stimulation.

Figure 5

(A and B) Flow cytometric analysis of (A) integrin αIIbβ3 activation (binding of JON/A-PE) and (B) degranulation-dependent P-selectin exposure in WT and Bin2fl/fl,Pf4Cre platelets in response to the indicated agonists (n = 4). Representative result of 3 independent experiments. Values are depicted as mean ± SD, and P values were calculated using the Mann-Whitney U test. **P < 0.01, ***P < 0.001. (C) Washed platelets were stimulated with the indicated agonists, and light transmission was recorded using a 4-channel aggregometer. Representative results of 3 independent experiments. (D) Phosphatidylserine exposure of platelets in response to the indicated agonists was measured by flow cytometry. n ≥ 5. Representative result of 3 independent experiments. Values are depicted as mean ± SD, and P values were calculated using the Mann-Whitney U test. **P < 0.01, ***P < 0.001.