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. 2020 Oct 19;130(11):6171–6186. doi: 10.1172/JCI135525

Figure 4. Detection of HTLV-1 plus-strand mRNAs in pregnant carrier–derived placental villous tissues.

Figure 4

RNA ISH with HTLV-1–specific probes targeting plus-strand mRNAs was used to stain the placental villous tissue sections of pregnant HTLV-1 carriers. The carriers were identical to those listed in Table 2. (A) ISH results are shown for each group of pregnant carriers (nos. 1, 9, and 16), and for a pregnant chronic ATL patient (no. 19). Viral mRNA was detected using DAB chromogen (brown). Boxes indicate regions shown at a higher magnification in adjacent lower panels. Data are representative of at least 5 nonserial tissue sections. Scale bars in lower and higher magnifications represent 200 μm and 50 μm, respectively. (B) Quantitative evaluation of ISH results. The total number of pX probe–positive foci in stained sections was counted by microscopy. Data are expressed as the mean ± SD from 5 nonserial tissue sections. The Mann-Whitney U test was performed to compare statistical differences between groups in subjects with HTLV-1–negative and –positive cord blood samples (nos. 7–12 vs. nos. 13–18).