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. 2020 Sep 28;130(11):5738–5755. doi: 10.1172/JCI138540

Figure 4. Expression of p53 and p21Wif1/Cip1 in nonsecreting gonadotroph/null cell, somatotroph, and lactotroph pituitary adenomas.

Figure 4

(A) TP53 (p53) and (B) CDKN1A (p21Wif1/Cip1) mRNA expression in nonsecreting gonadotroph/null cell (G/N), somatotroph (GH), and lactotroph (PRL) pituitary adenomas, normalized to ACTB. Results are presented as the mean ± SEM. *P ≤ 0.05 and **P ≤ 0.01, by 2-tailed, unpaired t test with Bonferroni’s correction. Protein expression of (C) p53 and (D) p21Wif1/Cip1 compared with Ponceau (P) staining in 3 different Western blot membranes with human adenoma sets E1, E2, and E3. (E and F) Quantitative presentation of (E) p53 and (F) p21Wif1/Cip1 in the 3 membranes after normalization to Ponceau, using ImageJ. Each experiment was analyzed separately. Results are presented as the mean ± SD. *P ≤ 0.05 and **P = 0.01, by 2-tailed, unpaired t test with Bonferroni’s correction.