. 2020 Jul 2;99(9):4505–4513. doi: 10.1016/j.psj.2020.06.015

Table 5.

Effect of lead nitrate on accumulation of Pb in the egg of laying hens.¹

Item²	Dietary Pb concentration (mg/kg)				P-value	SEM	Contrast P-value
Item²	3.20	33.20	63.20	93.20	P-value	SEM	L	Q
Albumen, mg/kg	0.20^c	0.27^c	0.51^b	0.72^a	<0.01	0.03	<0.01	0.02
Yolk, mg/kg	0.31^d	0.61^c	0.90^b	1.20^a	<0.01	0.04	<0.01	0.96
Albumen, %	38.99^a	30.50^b	36.32^a,b	37.63^a	<0.01	2.28	0.81	<0.01
Yolk, %	61.01^b	69.50^a	63.68^a,b	62.37^b	<0.01	2.28	0.81	<0.01

Abbreviation: Pb, plumbum.

Means within each row with different superscripts were significantly different (P < 0.05). SEM: standard error of the mean by ANOVA (n = 6); L: linear; Q: quadratic. Accumulation of Pb in egg = accumulation of Pb in albumen + accumulation of Pb in yolk. Albumen, % = accumulation of Pb in albumen/accumulation of Pb in egg. Yolk, % = accumulation of Pb in yolk/accumulation of Pb in egg.

Regression equation of the accumulation of Pb in albumen, y = −0.027 + 0.181x, R² = 0.923; regression equation of the accumulation of Pb in yolk, y = 0.014 + 0.296x, R² = 0.968.