Table 1.
Effect of time postinfection (PI), infection with Eimeria maxima (EM) or butyric acid glycerol esters (BE) addition in the diet on alpha diversity indices and beta diversity (PERMANOVA) in ileal content (IlC) and cecal content (CeC) and scrapings (ileal scraping [IlS] and cecal scraping [CeS]).
| Analysis | Pr = F |
|||
|---|---|---|---|---|
| ILC | IlS | CeC | CeS | |
| Observed OTU | ||||
| Time PI | 0.056 | 1.00 | 0.112 | 0.043 |
| EM | 0.139 | 0.049 | 0.583 | 0.507 |
| BE | 0.324 | 0.298 | 0.751 | 0.248 |
| Time PI × EM × BE | 0.161 | 0.170 | 0.614 | 0.204 |
| Shannon diversity index | ||||
| Time PI | 0.049 | 0.386 | 0.273 | 0.356 |
| EM | 0.139 | 0.453 | 0.729 | 0.166 |
| BE | 0.666 | 0.356 | 0.773 | 0.326 |
| Time PI × EM × BE | 0.134 | 0.513 | 0.737 | 0.486 |
| Faith's Phylogenetic Diversity (Richness) index | ||||
| Time PI | 0.065 | 0.133 | <0.001 | 0.094 |
| EM | 0.758 | 0.094 | 0.686 | 0.204 |
| BE | 0.042 | 0.453 | 0.729 | 0.225 |
| Time PI × EM × BE | 0.169 | 0.244 | 0.017 | 0.170 |
| Pielou's Evenness index | ||||
| Time PI | 0.019 | 0.299 | 0.729 | 0.043 |
| EM | 0.295 | 0.729 | 0.773 | 0.065 |
| BE | 0.951 | 0.686 | 0.488 | 1.000 |
| Time PI × EM × BE | 0.103 | 0.675 | 0.700 | 0.210 |
| PERMANOVA analysis (unweighted UniFrac) | ||||
| Time PI | 0.040 | 0.001 | 0.001 | 0.001 |
| EM | 0.191 | 0.670 | 0.472 | 0.553 |
| BE | 0.140 | 0.827 | 0.502 | 0.404 |
| Time PI × EM × BE | 0.194 | 0.001 | 0.001 | 0.001 |
Abbreviations: BE, birds receiving 0.25% butyric acid glycerol esters in a diet; BE + EM, birds receiving 0.25% BE in a diet and infected with EM, 7 and 10 day PI; C, control birds; EM, birds infected with Eimeria maxima (EM); OTU, operational taxonomic unit; PERMANOVA, permutational multivariate analysis of variance; PI, postinfection.