Table 3.
Sequences of primers used for quantitative real-time PCR.
| Gene symbol | Group | Gene name | Primer sequence (5ʹ-3ʹ) | Ta°C | Amplicon size (bp) | Reference | Function |
|---|---|---|---|---|---|---|---|
| CLDN1 | Tight junction protein | Claudin 1 | F-CTTCATCATTGCAGGTCTGTCAG R-AAATCTGGTGTTAACGGGTGTG |
60 | 103 | This study1 | Maintenance of intestinal barrier function |
| CLDN5 | Tight junction protein | Claudin 5 | F-GCAGGTCGCCAGAGATACAG R-CCACGAAGCCTCTCATAGCC |
61 | 162 | This study | Maintenance of intestinal barrier function |
| JAM2 | Tight junction protein | Junctional adhesion molecule 2 | F-AGACAGGAACAGGCAGTGCTAG R-ATCCAATCCCATTTGAGGCTAC |
60 | 135 | This study | Maintenance of intestinal barrier function |
| OCLD | Tight junction protein | Occludin | F- ACGGCAGCACCTACCTCAA R- GGGCGAAGAAGCAGATGAG |
60 | 123 | (Du et al., 2016) | Maintenance of intestinal barrier function |
| NaPi-IIb | Phosphorus transporter | Na-dependent Pi cotransporters, type IIb | F- CGGTCCGTTCACTCTGTTGC R- GAAGCCACGTTGCCTTTGTG |
60 | 165 | This study | Mediation of intestinal phosphate uptake |
| VDR | Vitamin D transporter | Vitamin D receptor | F- ACGCAGACATGGACACCGT R- GGACAGGTGAACATCGCTTTC |
60 | 193 | This study | Provides instructions for making vitamin D receptor |
| CALB1 | Calcium transporter | Calbindin 1 | F- GGCAGGCTTGGACTTAACACC R- GTCGGCAACACCTGAGCAAG |
60 | 105 | This study | Transport protein of calcium |
| ATP1A1 | Calcium transporter | ATPase Na+/K+ transporting subunit alpha 1 | F- GTCAACCCGAGGGATGCTAA R- ACTGCTACAATGGCACCCTG |
60 | 179 | (Kheravii et al. 2018) | Provides instructions for making Na+/K+ ATPase |
| ATP5A1W | Calcium transporter | ATP synthase subunit alpha | F- GGCAATGAAACAGGTGGCAG R- GGGCTCCAGCTTGTCTAAGTGA |
60 | 232 | This study | Synthesis of ATP |
| ATP13A4 | Calcium transporter | ATPase type 13A4 | F-CCAAAGCTCCTGCTAAATGC R-ATGCCTCCTGCTCTGACAGT |
61 | 178 | (Lee and Kim, 2018) | |
| ATP2A1 | Calcium transporter | ATPase, Ca++ transporting, cardiac muscle, fast twitch 1 | F-CGCTGTCAATCAGGACAAGA R-GTCGTTAAAGTGGCCGATGT |
61 | 250 | (Lee and Kim, 2018) | Catalyzes the hydrolysis of ATP and translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. |
| ATP2B1 | Calcium transporter | ATPase, Ca++ transporting, plasma membrane 1 | F-CTGGGCATGGGAACACTACT R-CACGACGTAATTCTCGCTCA |
60 | 169 | (Lee and Kim, 2018) | Catalyzes the hydrolysis of ATP and maintenance of intracellular calcium homeostasis |
| CASR | Calcium transporter | Calcium sensing receptor | F-CTGCGTGATTTGGCTCTACA R-GGCAAAGAAGAAGCAGATGG |
60 | 160 | (Lee and Kim, 2018) | Regulator of parathyroid hormone synthesis and secretion and systemic calcium homeostasis. |
| SLC8B1 | Calcium transporter | (Sodium/lithium/calcium exchanger), member B1 | F-CTTCGAGCTGAGCAACACTG R-CCCACACCCACCAGAATATC |
60 | 169 | (Lee and Kim, 2018) | Mediates mitochondrial calcium extrusion and mitochondrial calcium homeostasis |
| CACNA1A | Calcium channel | Calcium channel, voltage-dependent, P/Q type alpha 1A subunit | F-GCAGCGGGTCTATAAGCAGT R-GCGATGATGGTGGCTAAAAT |
60 | 198 | (Lee and Kim, 2018) | Provides instructions for making calcium channels |
| TPCN2 | Calcium channel | Two pore segment channel 2 | F-AGGTGCTGTGGTTCCTATGG R-GCCCACGGACTTGTGTATCT |
60 | 210 | (Lee and Kim, 2018) | Very little is known about the physiological functions but believe to form functional Ca2+-permeable channel |
| TPCN3 | Calcium channel | Two-pore calcium channel 3 | F-TGGGAATGGGAGTTCAAGAG R-CTGCCTCAAACATACGCTGA |
60 | 183 | (Lee and Kim, 2018) | Very little is known about the physiological functions but believe to form functional Ca2+-permeable channel |
| MUC-2 | Inflammatory genes | Mucin 2 | F- CCCTGGAAGTAGAGGTGACTG R- TGACAAGCCATTGAAGGACA |
60 | 143 | (Fan et al., 2015) | The physical and biological barrier protecting mucous epithelia |
| RPL4 | Housekeeping genes | Ribosomal protein L4 | F: TTATGCCATCTGTTCTGCC R: GCGATTCCTCATCTTACCCT |
60 | 235 | (Yang et al., 2013) | Component of the 60S subunit and encodes a ribosomal protein |
| β-actin | Housekeeping genes | β-actin | F-CTCTGACTGACCGCGTTACTCC R-CCATACCAACCATCACACCCTG |
60 | 175 | This study | Cytoskeletal structural protein, nucleotide, and ATP binding |
1
Primer sequencing for the gene was done in the present study and not that reported in a published paper.