Table 5.

Effect of necrotic enteritis, phytase, and calcium on phytate esters and inositol concentration (μmol/g DM) in the ileal digesta of broilers 29 D posthatch.

Effects				Inositol	IP3	IP4	IP5	IP6
	NE	Phy	Ca
2-Way interactions
NE∗Ca
-			Low	23.90	0.368	1.790	0.554c	3.52
-			High	13.50	1.982	7.194	5.143a	17.46
+			Low	36.02	0.245	1.425	0.278c	2.41
+			High	24.64	1.131	4.759	2.656b	10.53
Phy∗Ca
		500	Low	27.97	0.345b	1.885b,c	0.397	3.88b,c
		500	High	17.15	1.009b	4.681a,b	4.779	19.53a
		1,500	Low	31.95	0.269b	1.330c	0.435	2.04c
		1,500	High	20.99	2.103a	7.272a	3.021	8.45b
Main effects
NE	-			18.70b	1.175a	4.492	2.849	10.49a
	+			30.33a	0.688b	3.092	1.467	6.47b
Ca			Low	29.96a	0.307	1.608	0.416	2.96
			High	19.07b	1.556	5.976	3.900	13.99
SEM				5.88	0.72	2.32	1.38	4.15
P > f
NE				0.000	0.026	0.066	0.016	0.020
Phy				0.092	0.021	0.178	0.124	0.000
Ca				0.000	0.000	0.000	0.000	0.000
NE × Phy				0.094	0.273	0.875	0.571	0.456
NE × Ca				0.829	0.092	0.171	0.050	0.087
Phy × Ca				0.974	0.008	0.040	0.109	0.008
NE × Phy × Ca				1.000	0.279	0.587	0.343	0.322

^a,b,cMeans in the same column with different superscripts are significantly different (P < 0.05).

Phytase (Quantum Blue 5G).

2-way or 3-way interaction by Tukey.

Abbreviations: Ca, calcium; IP3, inositol triphosphate; IP4, inositol tetraphosphate; IP5, inositol pentaphosphate; IP6, inositol hexaphosphate; NE, necrotic enteritis; phy, phytase.