. 2020 Jul 3;99(10):4914–4928. doi: 10.1016/j.psj.2020.06.030

Table 7.

Effect of necrotic enteritis, phytase, and calcium on jejunal gene expression, day 16 posthatch.

Effects				CLDN1	CLDN5	JAM2D	MUC-2	NaPi-IIb	OCLD	VDR
	NE	Phy	Ca
2-Way interactions
Phy∗Ca
		500	Low	1.379^a	1.191	1.006	1.061	1.669	1.099	1.740^a
		500	High	0.773^c	1.234	1.120	1.002	1.188	1.029	0.940^b
		1,500	Low	0.864^c	0.997	0.999	0.965	1.285	1.115	0.930^b
		1,500	High	1.014^b	1.192	1.043	1.365	1.187	1.011	1.050^b
Main effects
NE	-			0.513^b	0.771^b	0.974	1.247^a	1.787^a	1.294^a	1.500^a
	+			1.502^a	1.537^a	1.110	0.949^b	0.878^b	0.832^b	0.830^b
Ca			Low	1.121	1.094	1.002	1.013	1.477	1.106	1.330
			High	0.893	1.213	1.081	1.184	1.187	1.020	1.000
SEM				0.45	0.46	0.21	0.26	0.36	0.21	0.55
P > f
NE				0.000	0.000	0.115	0.013	0.000	0.000	0.003
Phy				0.435	0.506	0.621	0.251	0.279	0.999	0.112
Ca				0.199	0.502	0.358	0.146	0.106	0.287	0.120
NE × Phy				0.872	0.394	0.072	0.341	0.760	0.451	0.599
NE × Ca				0.552	0.658	0.060	0.382	0.467	0.613	0.203
Phy × Ca				0.036	0.667	0.683	0.052	0.280	0.820	0.038
NE × Phy × Ca				0.137	0.756	0.142	0.504	0.533	0.988	0.934

^a,b,cMeans in the same column with different superscripts are significantly different (P < 0.05).

Phytase (Quantum Blue 5G).

2 or 3-way interaction by Tukey.

Abbreviations: Ca, calcium; CLDN1, claudin 1; CLDN5, claudin 5; JAM2D, junctional adhesion 2; MUC-2, mucin 2; NE, necrotic enteritis; NaPi-IIb, Na-dependent Pi cotransporters, type IIb; phy, phytase; OCLD, occluding; VDR, vitamin D.