Effect of delay in feed access for the first 48 h post-hatch on ileal level of mRNA for TJ genes: (A) occludin (OCLN), (B) zonula occluden-1 (ZO-1), (C) zonula occluden-2 (ZO-2), (D) claudin 1 (CLDN 1), (E) claudin 4 (CLDN 4), (F) claudin 5 (CLDN 5), (G) junctional adhesion molecule 2 (JAM2), and (H) junctional adhesion molecule 3 (JAM3). The expression level of e19 (−48 h) birds was set to 100% and the other values are presented as % of the −48 h data. Each value represents mean ± SE of 6 birds. Different letters (large characters for fed [FD] group and small characters for not fed [NFD] group) denote statistically significant (P < 0.05) differences between means within treatment. Stars denote statistically significant (P < 0.05) differences between treatment groups for each time point.