Table 1.

Gene-specific primers used for the analysis of mRNA levels using quantitative real-time RT-PCR (Untergasser et al., 2007).¹

Gene2	GenBank accession No.3	Forward primer (5’→3′)	Reverse primer (5’→3′)	Amplicon size (bp)
CLDN 1	NM_001013611	GGTGAAGAAGATGCGGATGG	TCTGGTGTTAACGGGTGTGA	139
CLDN 4	AY435420	ATCGCCCTGTCCGTCATC	ACCACGCAGTTCATCCACAG	137
CLDN5	NM_204201	AGGTGTCAGCCTTCATCGAC	CCAGGATGGAATCGTACACC	123
FABP2	NM_001007923	AGGCTCTTGGAACCTGGAAG	CTTGGCTTCAACTCCTTCGT	139
IgA	S40610	GAAGGTCTCCGTGGAGGATT	ACGTTGACGTGAGAGGCTTT	129
JAM 2	XM_015299112	CTGCTCCTCGGGTACTTGG	CCCTTTTGAAAATTTGTGCTTGC	135
JAM 3	XM_417876	CCAGAGTGTTGAGCTGTCCT	AGAATTTCTGCCCGAGTTGC	147
MUC2	NM_001318434	AAACAACGGCCATGTTTCAT	GTGTGACACTGGTGTGCTGA	127
OCLN	NM_205128	GATGGACAGCATCAACGACC	CTTGCTTTGGTAGTCTGGGC	142
pIgR	ENSGALT000000013534	CAAGGGAGTACGGAGCAAAC	CTTTGTCTCAGCGGTGCTTT	116
ZO-1	XM_015278975	GCCAACTGATGCTGAACCAA	GGGAGAGACAGGACAGGACT	141
ZO-2	NM_204918	TCAGCAACAGCAAGGTGAAG	GCACCCATGGCAGTAAGGTA	102

¹All primers used for expression analysis were designed using primer3 program (http://bioinfo.ut.ee/primer3-0.4.0/primer3/); (Untergasser et al., 2007).

²Abbreviations of the gene names are defined in text.

³Reference chicken gene sequences that contain the corresponding PCR products list.

⁴Transcript from Ensembl genome assembly (http://www.ensembl.org/Gallus_gallus/Info/Index), no prediction of this gene is available in GenBank.