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. 2020 Jul 2;99(10):4809–4813. doi: 10.1016/j.psj.2020.06.025

Figure 1.

Figure 1

Sensitivity verification of 3 kinds of technology in ILTV detection. (A) Sensitivity verification of RF-RAA in ILTV detection.1: Negative control; 2-7: Template concentration was 100-105 copies/μL. The results showed that the lowest detectable limit of RF-RAA was 10 copies/μL. B. Sensitivity verification of RFQ-PCR in ILTV detection.1: Negative control; 2-8: Template concentration was 100-106 copies/μL. The results showed that the lowest detectable limit of RFQ-PCR was 10 copies/μL. C. Sensitivity verification of conventional PCR in ILTV detection. M: Marker; 1-8: Template concentration was 107-100 copies/μL; 9: Negative control. The results showed that the lowest detectable limit of conventional PCR was 104copies/μL. Abbreviations: RF-RAA, real-time fluorescence-based recombinase-aided amplification; RFQ-PCR, real-time fluorescence-based quantitative PCR.