Table 1.

The comprehensive comparison of 3 techniques for ILTV detection.

Method	Primer design	Enzyme components	Instrument price	Response time	Reaction temperature	Cross-reaction	LDL	Degree of detection
RF-RAA	1 pair of primers, 1 probe	Recombinase, SSB, and polymerase	Cheap	20–30 min	37°C Constant	No	1-10 copies/μL	Real time quantitative, qualitative
RFQ-PCR	1 pair of primers	Taq polymerase	Expensive	1–2 hour	High and variable	No	10 copies/μL	Real time quantitative, qualitative
Conventional PCR	1 pair of primers	Taq polymerase	Expensive	2–3 hour	High and variable	No	104 copies/μL	Qualitative

Abbreviations: RF-RAA, real-time fluorescence-based recombinase-aided amplification; RFQ-PCR, real-time fluorescence-based quantitative PCR; SSB, single-strand DNA-binding protein.