Table 5.
ROS, TAOC, MDA, GPX, CAT, and SOD activity in the liver of broiler chickens fed with oxidized oil and different concentrations of quercetin.
Item | Treatment1 |
P-value, t-test | Linear | Quadratic | P-value | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Ctr | Ox | OxL | OxM | OxH | SEM | ||||||
11 D | ROS2 | 7.88 | 7.96∗,a | 7.87b | 7.91a,b | 7.92a,b | 0.018 | 0.014 | 0.426 | 0.016 | 0.018 |
TAOC3 | 1.20 | 1.25 | 1.18 | 1.20 | 1.33 | 0.024 | 0.382 | 0.200 | 0.161 | 0.298 | |
MDA4 | 0.55 | 1.15∗,a,b | 1.42a | 0.80b | 0.98b | 0.073 | <0.001 | 0.120 | 0.605 | 0.028 | |
GPX5 | 15.27 | 13.89a | 13.51a | 14.96a | 10.68b | 0.395 | 0.155 | 0.003 | 0.012 | 0.001 | |
CAT5 | 7.24 | 12.18∗ | 12.31 | 11.68 | 11.80 | 0.595 | 0.016 | 0.751 | 0.926 | 0.977 | |
SOD5 | 145.96 | 163.56∗ | 150.44 | 151.5 | 162.79 | 2.760 | 0.020 | 0.788 | 0.076 | 0.313 | |
18 D | ROS2 | 8.22 | 8.19 | 8.33 | 8.26 | 8.21 | 0.023 | 0.663 | 0.759 | 0.082 | 0.173 |
TAOC3 | 1.12 | 1.19a | 1.04b | 0.98b | 1.02b | 0.023 | 0.369 | 0.034 | 0.021 | 0.028 | |
MDA4 | 1.35 | 1.67a | 1.04b | 0.99b | 1.32a,b | 0.076 | 0.255 | 0.226 | 0.001 | 0.004 | |
GPX5 | 16.76 | 15.243a,b | 12.86c | 13.20b,c | 16.50a | 0.410 | 0.185 | 0.062 | 0.001 | 0.003 | |
CAT5 | 7.51 | 9.79 | 10.31 | 9.95 | 10.02 | 0.387 | 0.101 | 0.942 | 0.828 | 0.974 | |
SOD5 | 162.98 | 176.46a | 150.71c | 156.38b,c | 169.83a,b | 2.772 | 0.141 | 0.967 | 0.001 | 0.006 | |
42 D | ROS2 | 8.40 | 8.35 | 8.29 | 8.08 | 8.36 | 0.057 | 0.346 | 0.992 | 0.260 | 0.624 |
TAOC3 | 1.30 | 1.34b | 1.72a | 1.49a,b | 1.24b | 0.047 | 0.730 | 0.115 | 0.012 | 0.009 | |
MDA4 | 1.25 | 1.50 | 1.82 | 1.46 | 1.11 | 0.103 | 0.385 | 0.120 | 0.330 | 0.238 | |
GPX5 | 24.46 | 23.76 | 28.22 | 26.47 | 22.35 | 0.762 | 0.786 | 0.226 | 0.026 | 0.062 | |
SOD5 | 209.06 | 212.23 | 225.21 | 205.14 | 196.59 | 7.240 | 0.808 | 0.060 | 0.469 | 0.104 |
∗Means were significantly different compared with the control. Means within 4 treatments (Ox, OxL, OxM, and OxH) lacking a common superscript differ (P < 0.05).
Abbreviations: CAT, catalase; Ctr, control group; GPX, glutathione peroxidase; MDA, malondialdehyde; ROS, reactive oxygen species; SOD, superoxide dismutase; TAOC, total antioxidant capacity.
Ctr: basal diet; Ox: basal diet with oxidized oil; OxL: basal diet supplemented with oxidized oil and 200 ppm of quercetin; OxM: basal diet supplemented with oxidized oil and 400 ppm of quercetin; OxH: basal diet supplemented with oxidized oil and 800 ppm of quercetin.
ROS: log (fluorescence value of ROS).
TAOC: U/mg of protein.
MDA: nM/mg of protein.
GPX, SOD, CAT: U/mg of protein.