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. 2020 Jul 3;99(10):4892–4903. doi: 10.1016/j.psj.2020.06.028

Table 5.

ROS, TAOC, MDA, GPX, CAT, and SOD activity in the liver of broiler chickens fed with oxidized oil and different concentrations of quercetin.

Item Treatment1
P-value, t-test Linear Quadratic P-value
Ctr Ox OxL OxM OxH SEM
11 D ROS2 7.88 7.96∗,a 7.87b 7.91a,b 7.92a,b 0.018 0.014 0.426 0.016 0.018
TAOC3 1.20 1.25 1.18 1.20 1.33 0.024 0.382 0.200 0.161 0.298
MDA4 0.55 1.15∗,a,b 1.42a 0.80b 0.98b 0.073 <0.001 0.120 0.605 0.028
GPX5 15.27 13.89a 13.51a 14.96a 10.68b 0.395 0.155 0.003 0.012 0.001
CAT5 7.24 12.18∗ 12.31 11.68 11.80 0.595 0.016 0.751 0.926 0.977
SOD5 145.96 163.56∗ 150.44 151.5 162.79 2.760 0.020 0.788 0.076 0.313
18 D ROS2 8.22 8.19 8.33 8.26 8.21 0.023 0.663 0.759 0.082 0.173
TAOC3 1.12 1.19a 1.04b 0.98b 1.02b 0.023 0.369 0.034 0.021 0.028
MDA4 1.35 1.67a 1.04b 0.99b 1.32a,b 0.076 0.255 0.226 0.001 0.004
GPX5 16.76 15.243a,b 12.86c 13.20b,c 16.50a 0.410 0.185 0.062 0.001 0.003
CAT5 7.51 9.79 10.31 9.95 10.02 0.387 0.101 0.942 0.828 0.974
SOD5 162.98 176.46a 150.71c 156.38b,c 169.83a,b 2.772 0.141 0.967 0.001 0.006
42 D ROS2 8.40 8.35 8.29 8.08 8.36 0.057 0.346 0.992 0.260 0.624
TAOC3 1.30 1.34b 1.72a 1.49a,b 1.24b 0.047 0.730 0.115 0.012 0.009
MDA4 1.25 1.50 1.82 1.46 1.11 0.103 0.385 0.120 0.330 0.238
GPX5 24.46 23.76 28.22 26.47 22.35 0.762 0.786 0.226 0.026 0.062
SOD5 209.06 212.23 225.21 205.14 196.59 7.240 0.808 0.060 0.469 0.104

∗Means were significantly different compared with the control. Means within 4 treatments (Ox, OxL, OxM, and OxH) lacking a common superscript differ (P < 0.05).

Abbreviations: CAT, catalase; Ctr, control group; GPX, glutathione peroxidase; MDA, malondialdehyde; ROS, reactive oxygen species; SOD, superoxide dismutase; TAOC, total antioxidant capacity.

1

Ctr: basal diet; Ox: basal diet with oxidized oil; OxL: basal diet supplemented with oxidized oil and 200 ppm of quercetin; OxM: basal diet supplemented with oxidized oil and 400 ppm of quercetin; OxH: basal diet supplemented with oxidized oil and 800 ppm of quercetin.

2

ROS: log (fluorescence value of ROS).

3

TAOC: U/mg of protein.

4

MDA: nM/mg of protein.

5

GPX, SOD, CAT: U/mg of protein.