. 2020 Jul 3;99(10):4892–4903. doi: 10.1016/j.psj.2020.06.028

Table 9.

Relative mRNA expression of antioxidant- and inflammation-related genes in the broiler chicken ileal mucosa on day 11.

Item	Treatment¹						P-value, t-test	Linear	Quadratic	P-value
Item	Ctr	Ox	OxL	OxM	OxH	SEM	P-value, t-test	Linear	Quadratic	P-value
MUC2	1.00	0.15∗^,b	0.71^b	0.42^b	2.19^a	0.147	0.001	<0.001	0.080	<0.001
ZO1	1.03	0.22∗^,b	0.34^a,b	0.41^a	0.27^a,b	0.063	0.013	0.413	0.006	0.039
Claudin-2	1.00	0.71^b	1.53^b	0.67^b	3.34^a	0.23	0.441	0.001	0.059	0.001
Nrf2	1.00	0.37^b	0.65^b	0.81^b	2.10^a	0.157	0.102	<0.001	0.270	0.002
GCLM	1.65	0.67^b	0.66^b	0.57^b	1.35^a	0.142	0.192	0.002	0.011	0.002
CAT	1.00	0.50^b	0.87^b	0.96^b	2.30^a	0.146	0.121	<0.001	0.182	<0.001
SOD1	1.00	0.40^b	0.97^b	0.52^b	1.92^a	0.142	0.178	<0.001	0.154	<0.001
GPX2	1.00	1.01^b	1.40^b	1.28^b	2.21^a	0.131	0.970	0.003	0.494	0.018
GLRDX	1.15	0.90^b	1.55^b	1.17^b	2.39^a	0.150	0.412	0.002	0.525	0.008
TXN	1.14	0.77∗^,b	1.48^a	1.45^a	2.08^a	0.118	0.037	<0.001	0.486	0.002
HO-1	1.00	0.45^b	0.87^a,b	0.84^a,b	1.29^a	0.087	0.101	0.001	0.715	0.007
NOX2	1.03	0.90^a	1.10^a	0.74^a,b	0.49^b	0.068	0.397	0.012	0.372	0.033
IL-8	0.80	0.36∗^,c	0.51^b	0.47^b,c	0.65^a	0.033	0.024	<0.001	0.996	0.001

∗Means were significantly different compared with the control. Means within 4 treatments (Ox, OxL, OxM, and OxH) lacking a common superscript differ (P < 0.05).

Abbreviations: CAT, catalase; GCLM, glutamate–cysteine ligase modifier subunit; GLRDX, glutaredoxin; GPX2, glutathione peroxidase 2; HO-1, heme oxygenase-1; MUC2, mucin 2; Nrf2, nuclear factor erythroid 2–related factor 2; TXN, thioredoxin; SOD1, superoxide dismutase 1; ZO1, tight junction protein 1.

Ctr: basal diet; Ox: basal diet with oxidized oil; OxL: basal diet supplemented with oxidized oil and 200 ppm of quercetin; OxM: basal diet supplemented with oxidized oil and 400 ppm of quercetin; OxH: basal diet supplemented with oxidized oil and 800 ppm of quercetin.