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Primer sequences for real-time PCR assays.
| Gene | Gene bank ID | Primer sequence (5′-3′) | Length (bp) |
|---|---|---|---|
| Claudin-1 | NM_001013611.2 | F: TGATTGCTTCCAACCAGGCT | 89 |
| R: CACACGGCTCTCCTTGTCTA | |||
| Occludin | NM_205128.1 | F: ATCGCCTCCATCGTCTACATC | 90 |
| R: GCTGCACATGGCCAACAAG | |||
| ZO-1 | XM_413773.4 | F: TGGGCCTCACGGACTAAAAT | 118 |
| R: GTTTGCTCCAACAAGATAGTTTGG | |||
| GAPDH | NM_204305.1 | F: GGAAAGTCATCCCTGAGCTGAAT | 90 |
| R: GGCAGGTCAGGTCAACAACA |
Abbreviation: ZO-1, zonula occludens-1.
